Multicellular living organisms and unmodified parts thereof and – Method of using a plant or plant part in a breeding process...
Reexamination Certificate
2005-01-25
2005-01-25
Riley, Jezia (Department: 1637)
Multicellular living organisms and unmodified parts thereof and
Method of using a plant or plant part in a breeding process...
C800S277000, C800S278000, C536S023100, C536S024100, C435S440000, C435S468000, C435S320100, C435S419000
Reexamination Certificate
active
06846968
ABSTRACT:
The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human α galactosidase nucleotide sequences. The recombinant lysosomal enzymes produced in accordance with the invention may be used for a variety of purposes, including but not limited to enzyme replacement therapy for the therapeutic treatment of human and animal lysosomal storage diseases.
REFERENCES:
patent: 4956282 (1990-09-01), Goodman et al.
patent: 5082778 (1992-01-01), Overbeek et al.
patent: 5179023 (1993-01-01), Calhoun et al.
patent: 5401650 (1995-03-01), Desnick et al.
patent: 5580757 (1996-12-01), Desnick et al.
patent: 5597945 (1997-01-01), Jaynes et al.
patent: 5929304 (1999-07-01), Radin et al.
patent: WO 9010076 (1990-09-01), None
patent: WO 9710359 (1997-03-01), None
Murray G. J., et al., “Production of Recombinant Human Glucocerebrosidase in Plants”,Fed. of American Soc. for Experimental Biology, p. A1126, vol. 10, No. 6, Apr. 30, 1996, Journal, Fed. of American Soc. for Experimental Biology, Bethesda, Maryland.
Coppola G., et al., “Characterization of glycoylated and catalytically active recombinant human a-galactosidase A using a baculovirus vector”,Gene, pp. 197-203, 144 (1994), Elsevier Science B.V., Netherlands.
Hondred D. and Vierstra R. D., “Novel applications of the ubiquitin-dependent proteolytic pathway in plant genetic engineering”,Current Opinion in Biotechnology, pp. 147-151, vol. 3, (Apr. 1992), Current Biology Ltd.
Vierstra R. D., “Protein Degradation in Plants”,Annual Review of Plant Physiology and Plant Molecular Biology, pp. 385-410, vol. 44 (1993), Annual Reviews Inc., Palo Alto, California.
Kenward K D., et al., “Accumulation of type I fish antifreeze protein in transgenic tobacco is cold-specific”,Plant Molecular Biology, pp. 377-385, vol. 23, No. 2 (Oct. 1993), Kluwer Academic Publishers, Belgium.
Lee H. I. and Raikhel N. V., “Prohevein is poorly processed but shows enhanced resistance to a chitin-binding fungus in transgenic tomato plants”,Brazilian Journal of Medical and Biological Research, pp. 743-750, vol. 28 (Jul. 1995).
Erwin Robert L.
Grill Laurence K.
Kumagai Monto H.
Pogue Gregory P.
Turpen Thomas H.
Gallegos Thomas
Large Scale Biology Corporation
Riley Jezia
Robbins John C.
LandOfFree
Production of lysosomal enzymes in plants by transient... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Production of lysosomal enzymes in plants by transient..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of lysosomal enzymes in plants by transient... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3425574