Production of lysosomal enzymes in plant-based expression system

Details Production of lysosomal enzymes in plant-based expression system Production of lysosomal enzymes in plant-based expression system

1996-09-13

1999-07-27

Kemmerer, Elizabeth

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

Nonplant protein is expressed from the polynucleotide

800278, 800287, 800294, 800295, 8003173, 435 691, 435410, 435414, 435183, 435206, 4353201, 536 231, 536 232, 536 241, C12N 514, C12N 1552, C12N 1563

Patent

active

059293047

ABSTRACT:
The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants having recombinant expression constructs comprising human hGC and IDUA nucleotide sequences produced enzymatically active modified human glucocerebrosidase and human .alpha.-L-iduronidase. The recombinant lysosomal enzymes produced in accordance with the invention may be used for a variety of purposes, including but not limited to enzyme replacement therapy for the therapeutic treatment of human and animal lysosomal storage diseases.

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