Production of isoprenoids

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S252300, C536S023200, C536S023100

Reexamination Certificate

active

07572609

ABSTRACT:
The present invention relates to a process for the production of isoprenoids Coenzyme Q-10 by microorganisms. More particularly, the present invention relates to a process for increased production of Coenzyme Q-10 by microorganisms of the genusRhodobacter, preferably of the speciesR. sphaeroideswhich have been transformed with one or more gene(s) of the mevalonate (mev) operon from a different microorganism, preferably of the genusParacoccus, more preferably of the speciesP. zeaxanthinifaciens, whereby the mev operon is mutated leading to an increased coenzyme Q-10 production. Sequences carrying such a mutation as well as a microorganism carrying such a mutated mev operon are also included.

REFERENCES:
patent: 0 955 363 (1999-11-01), None
patent: 99/06586 (1999-02-01), None
patent: 02/099095 (2002-12-01), None
patent: WO2005000565 (2005-01-01), None
Chica et al. (Curr Opin Biotechnol. Aug. 2005; 16(4):378-84.
International Search Report of PCT/EP2005/008702, mailed Oct. 26, 2005.
Wilding et al. “Identification, Evolution, and Essentiality of the Mevalonate Pathway for Isopentenyl Diphosphate Biosynthesis in Gram-Positive Cocci” Journal of Bacteriology, vol. 182, No. 15, pp. 4319-4327, Aug. 2000.
Humbelin et al. “Genetics of Isoprenoid Biosynthesis inParacoccus zeaxanthinifaciens” Gene, vol. 297, No. 1-2, pp. 129-139, Sep. 4, 2002.

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