Production of isoleucine by escherichia coli having isoleucine a

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

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435115, 4351721, 435183, 4352528, C12P 1306, C12P 1308

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active

055344216

ABSTRACT:
The present invention provides strains of Escherichia coli which have an isoleucine-tRNA synthetase with an increased Km and no negative feedback inhibition of isoleucine production. These strains are effective for the production of isoleucine in high amounts.

REFERENCES:
patent: 4278765 (1981-07-01), Debabov et al.
patent: 4388405 (1983-07-01), Sano et al.
Iaccarino et al., "Isoleucine Auxotrophy as a Consequence of a Mutationally Altered Isoleucyl-transfer Ribonucleic Acid Synthetase," J Bacteriology, vol. 185 No. 2, pp. 527-537, 1971.
Mizukami et al., "Essential Role of Aspartokinase in L-Threonine Production by Escherichia coli W Mutants," Agric Biol. Chem. vol. 50, No. 4, pp. 1015-1018, 1986.
Roth et al., "Histidine Regulatory Mutants in Salmonella typhimurium", J. Mol. Biol., vol. 22, pp. 325-334, 1966.
R. P. Lawther, et al, Proc. Natl. Acad. Sci. USA, vol. 78, pp. 922-925 (1981).
R. P. Lawther, et al, J. of Bacteriology, vol. 149, pp. 294-298 (1982).

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