Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1985-04-26
1989-12-26
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4352402, 435320, 4351723, 935 34, C12P 2100, C12P 2102, C12N 1500, C12N 500, C12N 100
Patent
active
048898031
ABSTRACT:
Interferon .gamma. is produced in highly purified form (activity exceeding 10.sup.8 units/ml-day) by CHO cells cotransformed by a first plasmid bearing the genomic human interferon gene under the control of the SV40 early promoter, and a second plasmid bearing a DHFR gene under similar control. Methotrexate selection yielded a clone which was a particularly efficient producer. Production of interferon .gamma. may also be facilitated by a Harvey sarcoma virus enhancer sequence.
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Mory Yvez
Revel Michel
Rubinstein Menachem
Cooper Iver P.
Tanenholtz Alvin E.
Yeda Research & Development Co. Ltd.
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