Production of interferon-.beta. (IFN-.beta.) in E. coli

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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43525233, C12N 1522, C12N 121

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058144854

ABSTRACT:
Methods for the production of interferon-.beta. polypeptides by bacterial cells, by culturing cells capable of producing IFN-.beta. polypeptide under conditions that induce increased production of IFN-.beta. polypeptide. Such conditions include, for example, low potassium cation concentration and/or very low sodium cation concentration and/or specific pH ranges.

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Ausubel, et al. "Current Protocols in Molecular Biology", 1987, John Wiley & Sons. pp. 1.1.1 -1.1.3.

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