Production of insulin-like growth factor-1 in methylotrophic yea

Details Production of insulin-like growth factor-1 in methylotrophic yea Production of insulin-like growth factor-1 in methylotrophic yea

1993-02-25

1994-06-28

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

536 235, 536 234, 536 2351, 4353201, 435 691, 435 697, 435 698, 435 699, 43525423, C12P 2106, C12N 116, C12N 1500

Patent

active

053246390

ABSTRACT:
Insulin-like growth factor-1 (IGF-1), a naturally occurring, relatively short, single chain polypeptide, is prepared by growing methylotrophic yeast transformants containing in their genome at least one copy of a DNA sequence operably encoding IGF-1, in operational association with a DNA sequence encoding the S. cerevisiae alpha mating factor pre-pro sequence (including the proteolytic processing site: lys-arg), both under the regulation of a promoter region of a gene of a methylotrophic yeast, under conditions allowing expression of said DNA sequences, and secretion of IGF-1 into the culture medium.
Also disclosed are novel DNA fragments and novel recombinant yeast strains which are useful in the practice of the present invention.

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