Production of immobilized glucose isomerase

Details Production of immobilized glucose isomerase Production of immobilized glucose isomerase

1980-12-19

1982-08-10

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435 94, 435180, 435234, 435259, 435815, C12N 1102, C12P 1924, C12N 992

Patent

active

043439020

ABSTRACT:
An immobilized glucose isomerase is prepared by treating an aqueous suspension of cells of a glucose isomerase producing microorganism with a non-ionic surfactant that solubilizes glucose isomerase contained by the cells without solubilizing polysaccharides in the cells, separating the cells from the aqueous suspension, and adsorbing glucose isomerase from the resultant solution onto an ion exchange resin. By this process, contaminating polysaccharides are eliminated that inhibit adsorption of glucose isomerase on the ion exchange resin.

REFERENCES:
patent: 3948728 (1976-04-01), Roeschlau et al.
patent: 4113568 (1978-09-01), Fujita et al.
patent: 4144129 (1979-03-01), Gruber et al.
patent: 4263400 (1981-04-01), Ushiro

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