Production of human parathyroid hormone from microorganisms

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Calcitonin; related peptides

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

536 2351, C07K 736, C12N 1516

Patent

active

054202426

ABSTRACT:
The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. The invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast. Further the invention provides alternate polypeptides having parathyroid hormone activity, including PTH analogs, fragments and extensions, and provides alternate leader sequences and secretion signal sequences which can be used in the present invention. Finally, there are provided methods for purification of the secreted PTH hormone and/or derivatives.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4264731 (1981-04-01), Shine
patent: 4338397 (1982-07-01), Gilbert et al.
patent: 4366246 (1982-12-01), Riggs
patent: 4394443 (1983-07-01), Weissman et al.
patent: 4425437 (1984-01-01), Riggs
patent: 4468464 (1984-08-01), Cohen et al.
patent: 4532207 (1985-07-01), Brewer et al.
patent: 4546082 (1985-10-01), Kurjan et al.
patent: 4588684 (1986-05-01), Brake
patent: 4904584 (1990-02-01), Shaw
patent: 5010010 (1991-04-01), Gautvik et al.
Kronenberg et al., "Studies of Parathyroid Hormone Secretion Using Recombinant DNA Technology," Endocrine Control of Bone and Calcium Metabolism, 217 (1984).
Born et al., (Abstract) "Expression of Human Preproparathyroid Hormone in E. coli and Yeast," Calcified Tissue International, vol. 35, 679 (1983).
Born et al., (Abstract) "Expression of Human Preproparathyroid Hormone in E. coli and Yeast," Calcified Tissue International, vol. 36 (Suppl. 2), 287 (1984).
Hendy et al., "Nucleotide Sequence of Cloned cDNAs Encoding Human Preproparathyroid Hormone," Proc. Natl. Acad. Sci. USA, vol. 78, 7365 (1981).
Breyel et al., "Synthesis of Mature Human Parathyroid Hormone in Escherichia coli," Third European Congress on Biotechnology, Vo. III, 363 (1984).
Breyel et al., (Abstract) "Synthesis of Mature Human Parathyroid Hormone in Escherichia coli," Calcified Tissue International, vol. 36 (Suppl. 2), 297 (1984).
Born et al., (Abstract) "Expression And Processing of Human Preproparathyroid Hormone in Escherichia coli," Experientia, vol. 39, 659 (1983).
Hendy, (Abstract) "Cloning of Human Parathyroid Hormone mRNA and Gene," Calcified Tissue International, vol. 36, 286 (1984).
McDevitt et al., (Abstract) "Isolation of Human Parathyroid Hormone Genes," Calcified Tissue International, vol. 31, 74 (1980).
Gordon et al., (Abstract) "Molecular Cloning and Structural Analysis of Near Full-Length DNA Complementary to the mRNA Coding for Bovine Parathyroid Hormone," Fed. Proc., 39, 947 (1980).
Vasicek et al., "Nucleotide Sequence of the Human Parathyroid Hormone Gene," Proc. Nat'l. Acad. Sci., vol. 80, 2127 (1983).
Kronenberg et al., "Cloning and Nucleotied Sequence of DNA Coding for Bovine Preproparathyroid Hormone," Proc. Natl'l Acad. Sci., vol. 76, 4981 (1979).
Sung et al., "Hybrid Gene Synthesis: Its Application to the Assembly of DNA Sequences Encoding the Human Parathyroid Hormones and Analogues," Biochem. Cell Biol., vol. 64, 133 (1986).
Keutmann et al., "Complete Amino Acid Sequence of Human Parathyroid Hormone," Biochemistry, vol. 17, 5723 (1978).
Naylor et al., "Human Parathyroid Hormone Gene (PTH) is on Short Arm of Chromosome II," Somatic Cell Genetics, vol. 9 (1983).
Kronenberg et al., (Abstract) "Structural Analysis of the Human Parathyroid Hormone Gene," Calcified Tissue International, vol. 33, 322 (1981).
Hellerman et al., "Secretion of Human Parathyroid Hormone from Rat Pituitary Cells Infected with a Recombinant Retrovirus Encoding Preproparathyroid Hormone," Proc. Nat'l. Acad. Sci., vol. 81, 5340 (1984).
Brake et al., Alpha-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae, Mar. 28, 1984.
Cantrell et al., cDNA Cloning, Expression, and Activity of Human Granulocyte-Macrophage Colony-Stimulating Factor, 1987.
Arnst, J. F., Efficient Secretion and Processing of Heterologous Protein in Saccharomyces cerevisiae is mediated solely by the Pre-Segment of Alpha-Factor Precursor, DNA 7 (1988).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Production of human parathyroid hormone from microorganisms does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Production of human parathyroid hormone from microorganisms, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of human parathyroid hormone from microorganisms will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-362836

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.