Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2005-08-30
2005-08-30
Leffers, Gerry (Department: 1636)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S069100, C435S069700, C435S252330, C435S005000, C530S300000, C530S355000, C530S412000
Reexamination Certificate
active
06936455
ABSTRACT:
The present invention relates to a process for the recombinant production of a desired heterologous polypeptide with a clearly defined homogenous N-terminus in a bacteria host cell. A fusion comprising a peptide with the autoproteolytic activity of an autoprotease Nproof a pestivirus and the heterologous polypeptide is initially expressed in the form of cytoplasmic inclusion bodies in the host cell, the inclusion bodies are isolated and subsequently treated so that the desired heterologous polypeptide is cleaved autoproteolytically by the Nproactivity of the fusion protein.
REFERENCES:
patent: 5149783 (1992-09-01), Sommergruber et al.
patent: 2301698 (1999-03-01), None
patent: 234888 (1987-09-01), None
patent: 321973 (1989-06-01), None
patent: WO 98/49326 (1998-05-01), None
patent: WO 99/10483 (1999-03-01), None
patent: WO 99/10503 (1999-03-01), None
Collins-Racie et al., Bio-Technology, vol. 13, No. 9, 982-987 (1995).
Muyldermans et al., Virus Genes, vol. 13, No. 2, 135-142 (1996).
Ruemenapf et al., J of Virology, vol. 72, No. 3, 2544-2547 (1998).
Stark et al., J. of Virology, vol. 67, No. 12, 7088-7095 (1993).
Wiskerchen et al., J. of Virology, vol. 65, No. 8, 4508-4514 (1991).
Bachmann, “Section G. Strains and Useful Strain Constructions, 72. Derivations and Genotypes of Some Mutant Derivatives ofEscherichia coliK-12”,Escherichia coli and Salmonella Typhimurium: Cellular and Molecular Biology, vol. 2, Neidhardt, Ed., American Society for Microbiology, Washington, DC, pp. 1190-1219 (1987).
Bachmann, “Section A. The Genome, 53. Linkage Map ofEscherichia coliK-12, Edition 7”,Escherichia coli and Salmonella Typhimurium: Cellular and Molecular Biology, vol. 2, Neidhardt, Ed., American Society for Microbiology, Washington, DC, pp. 807-876 (1987).
Jensen, “TheEscherichia coliK-12 ‘Wild Types’ W3110 and MG1655 Have an rph Frameshift Mutation That Leads to Pyrimidine Starvation Due to Low pyrE Expression Levels”,J Bacteriol, vol. 175, No. 11, pp. 3401-3407 (1993).
Rudolph, “Renaturation of Recombinant, Disulfide-Bonded Proteins from ‘Inclusion Bodies’”,Modern Methods in Protein-and Nucleic Acid Research, Tschesche, Ed., pp. 149-171 (1990).
Rudolph, “Chapter 10: Successful Protein Folding on an Industrial Scale”,Protein Engineering: Principles and Practice, Cleland and Craik, Eds., John Wiley & Sons, Inc., pp. 283-298 (1996).
Rudolph, Bohm, Lilie and Jaenicke, “3: Folding Proteins”,Protein Function. A Practical Approach, Second Edition, Creighton, Ed., pp. 57-99 (1997).
Varnavski and Khromykh, “Noncytopathic Flavivirus Replicon RNA-Based System for Expression and Delivery of Heterologous Genes”,Virology, vol. 255, No. 2, pp. 366-375 (1999).
Knippers, Rolf, Molekulare Genetic, 6thEdition, Georg Thieme Verlag, Stuttgart, New York (Monograph) (1995).
Studier, F.W., Methods in Enzymology 185, pp. 60-89 (1990).
Abstract of oral presentation No. 4 V2 at the Jahrestagung Gesellschaft für Virologie, Hamburg, Oct. 13, 2003 (1997).
Poster Abstract No. 7P33 at the Jahrestagung Gesellschaft für Virologie, Regensburg, Mar. 2, 2005., (1998).
Poster Abstract No. P2-46 at the Fifth International Symposium on Positive Strand RNA Viruses, St. Petersburg, Florida, May 23-28 (1998).
E. F. Rossomando, Methods in Enzymology, vol. 182: Guide to Protein Purification, Academic Press Inc., pp. 309-392 (1990).
Knauseder Franz
Stempfer Günter
Windisch Jörg
Furman Diane E.
Leffers Gerry
Marvich Maria
Sandoz AG
Thallemer John D.
LandOfFree
Production of heterologous proteins using an Npro... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Production of heterologous proteins using an Npro..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of heterologous proteins using an Npro... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3514882