Production of heterologous proteins in plants and plant cells

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 691, 435 701, 800205, 800250, 800255, 800DIG42, 800DIG43, 530363, 5303873, 47 58, C12N 1563, C12N 1582, A01H 500, C07K 14765

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056503075

ABSTRACT:
This invention provides for a method to produce excreted and correctly processed heterologous protein-material in a plant host. The method comprises the step of transforming a plant host using a recombinant polynucleotide, which comprises the DNA sequences encoding the fully processed heterologous protein material, directly preceded by a DNA sequence coding for a plant signal peptide, and regulatory sequences necessary for the plant host to express the heterologous gene construct, resulting in excretion of the heterologous protein from the cell and correct cleavage of the N-terminal signal peptide, so that the mature heterologous protein produced by the plant host is identical to the corresponding protein produced in its authentic host.

REFERENCES:
patent: 4956282 (1990-09-01), Goodman et al.
C. Dorel et al., J. Cell Biol., vol. 108 (Feb. 1989) pp. 327-337.
G. Iturriaga et al., The Plant Cell, vol. 1 (Mar. 1989) pp. 381-390.
C. Dorel et al. (Abstract) Plant Physiology, vol. 86 (4 Suppl.) (88) p. 84.

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