Production of heterologous polypeptides from freshwater...

Details Production of heterologous polypeptides from freshwater... Production of heterologous polypeptides from freshwater...

2005-03-01

2005-03-01

Carlson, Karen Cochrane (Department: 1653)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

Reexamination Certificate

active

06861245

ABSTRACT:
A method is provided for screeningCaulobactersuitable for use as host organisms for secretion of heterologous polypeptides. SuchCaulobacterhave a transport protein homologous to one of the type I transport proteins known inC. crescentus. DNA constructs are also provided which code for a chimeric protein of which the C-terminus is a secretion signal of aCaulobactersurface layer protein, other than fromC. crescentus. Bacterial cells containing, or which express such DNA constructs and which may secrete the resulting protein, are also provided.

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