Production of guar alpha-galactosidase by hosts transformed with

Details Production of guar alpha-galactosidase by hosts transformed with Production of guar alpha-galactosidase by hosts transformed with

1991-06-26

1994-03-22

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 691, 435 698, 43525231, 4352523, 4352404, 4351723, 4353201, 43525411, 4352542, 43525421, 43525423, 4352543, 536 232, 536 236, 935 14, 935 23, 935 28, 935 29, 935 37, 935 38, 935 55, C12N 940, C12N 1556, C12N 1580, C12N 1574

Patent

active

052963659

ABSTRACT:
The gene encoding an alpha-galactosidase from guar seed has been isolated and identified. It has been expressed in yeasts, e.g. Saccharomyces, Kluyveromyces and Hansenula, in bacteria, e.g. Bacillus, and in plants, e.g. Nicotiana. The resulting alpha-galactosidase is able to decrease the galactose content of galactomannans such a guar gum by splitting off 1-6 linked alpha-D-galactopyranosyl units attached to a main chain of 1-4 lined beta-mannopyranosyl units. Thus a commercially feasible process becomes available for producing alpha-galactosidases suitable for providing galactomannans having improved properties, which can meet the increasing demand for good quality polysaccharides. It has further been demonstrated that other alphagalactosidases, which show a positive immunological cross-reaction with an antibody raised against the alpha-galactosidase from guar, are also effective in decreasing the galactose content of galactomannans such as guar.

REFERENCES:
patent: 4548904 (1985-10-01), Kent et al.
Wallace et al. "The use of synthetic oligonucleotides as . . . " Nuc. Acids Res. 9:879-894 (Feb. 1981).
Maniahs et al. "Molecular Cloning: A Laboratory Manual" Cold Spring Harbor Laboratory, pp. 187-198, 211-246, 390-401 (Jul. 1982).
Bitter et al. "Expression of heterologous genes in . . . " Gene 32:263-274 (Dec. 1984).

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