Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2007-05-08
2007-05-08
McElwain, Elizabeth F. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C800S288000
Reexamination Certificate
active
10723083
ABSTRACT:
The present invention discloses a method of producing granulocyte-macrophage colony stimulating factor (GM-CSF) in a plant comprising, transforming the plant with a genetic construct comprising a regulatory region functional in the plant, operably associated with a GM-CSF coding sequence, or a fragment or a derivative thereof, operably associated with a transcriptional terminator, and expressing the GM-CSF. Also disclosed are transgenic plants, seeds and cells comprising GM-CSF coding sequences and plant optimized GM-CSF coding sequences.
REFERENCES:
patent: 5677474 (1997-10-01), Rogers
patent: 5889189 (1999-03-01), Rodriguez
patent: 6288302 (2001-09-01), Yu et al.
Kaushansky et al., Hematopoietic activity of granulocyte/macrophage colony-stimulating factor is dependent upon two distinct regions of the molecule: Functional analysis based upon the activities of interspecies hybrid growth factors,PNAS,86:1213-1217 1989.
Schnepf et al.,Bacillus thuringiensisand Its Pesticidal Crystal Proteins, Microbiology and Molecular Biology Reviews, 62:775-806, 1998.
Sardana et al., Biological activity of human granulocyte-macrophage colony stimulating factor is maintained in a fusion with seed glutelin peptide, Transgenic Research 11:521-531, 2002.
Aoyama and Chua, “A glucocortocoid-mediated transcriptional induction system in transgenic plants,” The Plant Journal vol. 11, No. 3, pp. 605-612 (1997).
Brandstatter, I. and Kieber, J.J., “Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinin in Arabidopsis,” The Plant Cell vol. 10, pp. 1009-1019 (1998).
Burgess, A.W., et al. “Purification and properties of bacterially synthesized human granulocyte-macrophage colony stimulating factor,” Blood, vol. 69, pp. 43-51 (1987).
Caddick et al, “An ethanol inducible gene switch for plants used to manipulate carbon metabolism,” Nature Biotech. vol. 16, pp. 177-180 (1998).
Cantrell, M.A., et al. “Cloning, sequence, and expression of a human granulocyte/macrophage colony-stimulating factor,” Proc Natl Acad Sci USA vol. 82, pp. 6250-6254 (1985).
Cheng, X et al., “Rice transformation by Agrobacterium infection,” In: Recombinant Proteins from Plants: Production and Isolation of Clinically Useful Compounds. (eds. C. Cunningham and A.J.R. Porter) Humana Press, pp. 1-9 (1998).
Cheng et al., “Agrobacterium-transformed rice plants expressing synthetic CrylA(b) and CrylA(c) genes are highly toxic to striped stem borer and yellow stem borer,” Proc Natl Acad Sci USA vol. 95, pp. 2767-2772 (1998).
Denecke et al, “Protein secretion in plant cells can occur via a default pathway,” The Plant Cell, vol. 2, pp. 51-59 (1990).
Ernst, J.F., et al. “O-glycosylation and novel processing events during secretion of alpha-factor/GM-CSF fusions bySaccharomyces cerevisiae,” Bio/Technology, vol. 5, pp. 831-834 (1987).
Gatz, C., “Chemical Control of Gene Expression,” Ann. Rev. Plant Physiol. Plant Mol. Biol. vol. 48, pp. 89-108 (1997).
Jaeger, G.D, et al. “Boosting heteriogous protein production in transgenic dicotyledonous seeds using Phaseolus vulgaris regulatory sequences,” Nature biotechnology, vol. 20, pp. 1265-1268 (2002).
James, E.A., et al., “Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells,” Protein Express Purif, vol. 19, pp. 131-138 (2000).
Kakimoto, T., “CKl1, a histidine kinase homolog implicated in cytokinin signal transduction,” Science, vol. 274, pp. 982-985 (1996).
Kaushansky, K., et al. “Role of carbohydrate in the function of human granulocyte-macrophage colony-stimulating factor,” Biochemistry vol. 26, pp. 4861-4867 (1987).
Kitamura, T., et al., “Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin,” J Cellular Physiol, vol. 140, pp. 323-334 (1989).
Lee, F., et al. “Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells,” Proc Natl Acad Sci USA vol. 82, pp. 4360-4364 (1985).
Metcalf, D, “Control of granulocytes and macrophages: Molecular, cellular, and clinical aspects,” Science vol. 254, pp. 529-533 (1991).
Moonen, P., et al. “Increased biological activity of deglycosylated recombinant human granulocyte/macrophage colony-stimulating factor produced by yeast or animal cells,” Proc Natl Acad Sci USA vol. 84, pp. 4428-4431 (1987).
Murray et al., “Codon usage in plant genes,” Nuc Acids Res. vol. 17, pp. 477-498 (1989).
Okamoto, M., et al. “Amplification and high-level expression for human granulocyte-macrophage colony-stimulating factor in human lymphoblastoid Namalwa cells,” Bio/Technology, vol. 8, pp. 550-553 (1990).
Quesniaux and Jones. “Granulocyte-macrophage colony-stimulating factor,” In: The Cytokine Handbook, (ed. Angus T.W.) Academic Press pp. 637-670 (1998).
Saalbach, I., et al. “High-level expression of a single-chain Fv fragment (scFv) antibody in transgenic pea seeds.” J. Plant Physiol. vol. 158, pp. 529-533 (2001).
Salter et al, “Characterisation of the ethanol-inducible alc gene expression system for transgenic plants,” The Plant Journal vol. 16, No. 1, pp. 127-132 (1998).
Sardana et al., “Construction and rapid testing of synthetic and modified toxin gene sequences CrylA (b&c) by expression in maize endosperm culture,” Plant Cell Reports vol. 15, pp. 677-681 (1996).
Sardana R, et al. “Biological activity of human granulocyte macrophage colony stimulating factor is maintained in a fusion with seed glutelin peptide,” Transgenic Research vol. 11, No. 5, pp. 521-531 (2002).
Stoger, E., et al. “Cereal crops as viable production and storage systems for pharmaceutical ScFv antibodies,” Plant Mol Biol., vol. 42, pp. 583-590 (2000).
Tobias et al., “The N-end rule in bacteria,” Science, vol. 254, pp. 1374-1377 (1991).
Ulmasov, T., et al., “Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements,” The Plant Cell, vol. 9, pp. 1963-1971 (1997).
Varshavsky, “The N-end rule: functions, mysteries, uses,” Proc. Natl. Acad. Sci USA, vol. 93, pp. 12142-12149 (1996).
Vitale, A., et al., “The role of endoplasmic reticulum in protein synthesis, modification and intracellular transport,” Journal of Experimental Botany, vol. 44, No. 266, pp. 1417-1444 (1993).
Wong, G.G., et al. “Human GM-CSF: Molecular cloning of the complementary DNA and purification of the natural and recombinant proteins,” Science, vol. 228, pp. 810-815 (1985).
Zheng, Z., et al. “5′distal and proximal cis-acting regulator elements are required for developmental control of a rice seed storage protein glutelin gene,” The Plant Journal, vol. 4, No. 2, pp. 357-366 (1993).
Zheng, Z.W., et al. “The bean seed storage protein beta-phaseolin is synthesized, processed, and accumulated in the vacuolar type-II protein bodies of transgenic rice endosperm,” Plant Physiol vol. 109, pp. 777-786 (1995).
Altosaar Illimar
Dudani Anil
Ganz Peter
Sardana Ravinder
Tackaberry Eilleen
McElwain Elizabeth F.
Wilmer Cutler Pickering Hale and Dorr LLP
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