Production of glucuronic acid using myo-inositol oxygenase...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Reexamination Certificate

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C435S069100, C435S252300, C435S136000

Reexamination Certificate

active

07923231

ABSTRACT:
A method is disclosed for increasing the specific activity of myo-inositol oxygenase. The method includes incubating a mixture including myo-inositol oxygenase and a non-sulfur containing reductant under conditions effective to increase the specific activity of the myo-inositol oxygenase. Also disclosed are methods for producing D-glucuronic acid and glucurono-γ-lactone comprising incubating a mixture including myo-inositol, myo-inositol oxygenase, and oxygen under conditions effective to form 5 grams D-glucuronic acid per liter of the mixture to 400 grams D-glucuronic acid per liter of the mixture. Glucurono-γ-lactone can be produced from the D-glucuronic acid product. Also disclosed are organisms and nucleic acids suitable for use in such methods.

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