Production of gamma linolenic acid by a Δ6-desaturase

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S281000, C435S320100, C435S419000

Reexamination Certificate

active

10029756

ABSTRACT:
Linoleic acid is converted into γ-linolenic acid by the enzyme Δ6-desaturase. The present invention is directed to isolated nucleic acids comprising the Δ6-desaturase gene. More particularly, the isolated nucleic acid comprises the promoter, coding region and termination regions of the Δ6-desaturase gene. The present invention provides recombinant constructions comprising the Δ6-desaturase coding region in functional combination with heterologous regulatory sequences. The nucleic acids and recombinant constructions of the instant invention are useful in the production of GLA in transgenic organisms.

REFERENCES:
patent: WO 99/27111 (1999-06-01), None
Broun et al, Science 282: 131-133, Nov. 13, 1998.
Van de Loo et al, Proc Natl. Acad Sci, USA, 92: 6743-6747, Jul. 1995.
Doerks et al, TIG 14(6): 248-250, Jun. 1998.
Smith et al, Nature Biotechnology 15: 1222-1223, Nov. 1997.
Brenner, S. E., TIG 15(4): 132-133, Apr. 1999.
Bork et al, TIG 12(10): 425-427, Oct. 1996.
De Luca, V, AgBiotech News and Information 5(6): 225N-229N, 1993.

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