Production of flavonoids by recombinant microorganisms

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...

Reexamination Certificate

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C435S041000, C435S069100, C435S252800, C536S023740

Reexamination Certificate

active

11178912

ABSTRACT:
Methods and compositions are provided for production of flavonoids in microbial hosts. The compositions comprises a set of genes which encode for enzymes involved in one or more steps in the biosymthetic pathway for the conversion of phenylpropanoids to various flavonoids. The method comprises the steps of introducing the set of genes into a heterologous host cell, allowing growth of the cells in a suitable medium such that the expression of the genes results in production of enzymes. When specific substrate(s) is/are provided to the transformed cell, the enzymes act on the substrate(s) to produce the desired flavonoids.

REFERENCES:
patent: 2005/0208643 (2005-09-01), Schmidt-Dannert et al.
Hwang, et al. Applied and Environ Microbiol, May 2003, pp. 2699-2706.
Honda et al. ( Plant Phys Biochem. ( 2002, 40, 955-962).
Hwang, et. al, Production of Plant-Specific Flavanones byEscherichia coliContaining an Artificial Gene Cluster, Applied and Environmental Microbiology, May 2003, pp. 2699-2706.
Ralston, et. al., Partial Reconstruction of Flavonoid and Isoflavonoid Biosynthesis in Yeast Using Soybean Type I and Type II Chalcone Isomerases, Plant Physiology, Preview Section, Jan. 2005, pp. 1-14.
Schijlen, et. al., Modification of flavonoid biosynthesis in crop plants, Phytochemistry, Sep. 2004, vol. 65, pp. 2631-2648.

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