Production of enzymatically active recombinant carboxypeptidase

Details Production of enzymatically active recombinant carboxypeptidase Production of enzymatically active recombinant carboxypeptidase

1997-01-13

1999-09-07

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

530415, 530416, C12N 950

Patent

active

059486686

ABSTRACT:
The subject invention provides a method of producing enzymatically active CPB which comprises treating a recombinant cell containing DNA encoding ProCPB, so that the DNA directs expression of the ProCPB, recovering from the cell the ProCPB so expressed, treating the recovered ProCPB under conditions permitting folding of the ProCPB, subjecting the folded ProCPB to enzymatic cleavage to produce enzymatically active CPB and purifying the enzymatically active CPB.

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