Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1988-10-14
1992-03-10
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352521, 435822, C12N 300, C12N 120
Patent
active
050949543
ABSTRACT:
Disclosed here is a novel method for producing bacterial endospores which can be used as biocontrol agents for nematodes. Advantageously, the novel method does not require that bacteria be grown on living nematodes. Instead, explanted nematode tissue is used as the growth medium for the bacteria. The invention further concerns novel compositions comprising spores produced using the disclosed process.
REFERENCES:
Sasser, J. N. (1980) "Root-Knot Nematodes: A Global Menace to Crop Production", Plant Disease 64(1):36-41.
Sayre, R. M. (1980) "Promising Organisms for Biocontrol of Nematodes", Plant Disease 64:527-532.
Stirling, G. R. (1984) "Biological Control of Meloidogyne javanica with Bacillus penetrans", Phytopathology 74:55-60.
Sayre, R. M., M. P. Starr, A. M. Golden, W. P. Wergin, and B. Y. Endo (1988) "Comparison of Pasteuria penetrans from Meloidogyne incognita with a Related Mycelial and Endospore-Forming Bacterial Parasite from Pratylenchus brachyrus", Proc. Helminthol. Soc. Wash. 55(1):28-49.
Starr, M. P. , and R. M. Sayre (1988) "Pasteuria thornei sp. nov. and Pasteuria penetrans sensu stricto emend., Mycelial and Endospore-Forming Bacteria Parasitic, Respectively, on Plant-Parasitic Nematodes of the Genera Pratylenchus and Meloidogyne", Ann. Inst. Pasteur/Microbiol. 139:11-31.
Verdejo, S., and R. Mankau (1986) "Culture of Pasteuria penetrans in Meloidogyne incognita on Oligoxenic Excised Tomato Root Culture", J. Nematology 18:365.
Reise, R. W., K. J. Hackett, R. M. Sayre, and R. N. Huettel (1988) "Factors Affecting Cultivation of Three Isolates of Pasteuria sp.", Abstracts of the 27th Annual Meeting, Society of Nematologists, p. 75.
Wolf, K., "Cold-Blooded Vertebrate Cell and Tissue Culture", Methods in Enzymology, vol. LVIII, Jacoby and Pastan, Ed., pp. 466-477, 1979.
Stirling, G. et al., "Attachment of Pasteuria penetrans spores to the Cuticles of Root-Knot nematodes", Revue Nematol. 9(3): 251-260 (1986).
Starr et al., "Pasteuria Thornei sp. nov. and Pasteuria penetrans sensu stricto Emend., Mycelial and Endospore-Forming Bacteria Parasitic, Respectively, on Plant-Parasitic Nematodes of the Genera Pratylenchus and Meloidogyne", Ann. Inst. Pasteur/Microbiol. (1988) 139, pp. 11-31.
Bhattacharya et al., "Pasteuria penetrans, A Pathogen of the genus Heterodera, its Effect on Nematode Biology and Control", Indian J. Nematol. 18(1): pp. 61-70 (1988).
Cox Robert J.
Previc Edward P.
Knode Marian
Robinson Douglas W.
University of Florida
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