Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2006-06-29
2010-06-15
Raghu, Ganapathirama (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S069100, C435S183000, C435S252300, C435S320100, C536S023200
Reexamination Certificate
active
07736878
ABSTRACT:
Recombinant forms of DNA sequences for CPD glycosylases, including the bacteriophage T4 gene denV, are described that are capable of expression at high levels. Active CPD glycosylases can be recovered from inclusion bodies resulting from the high expression using, for example, a homogenization process which employs stream mixing, and the active proteins can be used in, for example, topical formulations for treatment of photosensitive diseases. Stream mixing can also be used to solubilize inclusion bodies containing proteins other than CPD glycosylases.
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Estis Leonard F.
Kraus Eliyahu
Yarosh Daniel B.
Applied Genetics Incorporated Dermatics
Raghu Ganapathirama
Yang Yongzhi
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