Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1981-11-12
1988-01-12
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
4351723, 435235, 435236, 435239, 435253, 435847, 435327, 536 27, 935 12, 935 19, 935 21, 935 18, 935 22, 935 56, 935 65, 935 73, C12P 1934, C12N 1500, C12N 120, C12N 100
Patent
active
047191775
ABSTRACT:
Methods for producing RNA viral cDNA, such as poliovirus ds cDNA, products thereof, and uses thereof, are described. Poliovirus cDNA is produced, for example, by reverse transcribing poliovirus RNA and subsequently inserting the poliovirus cDNA into bacterial plasmids by genetic-engineering techniques. Transformed bacteria are then cloned and cultured to produce replicated chimeric plasmids containing the cDNA poliovirus. Such poliovirus cDNA is useful in assaying for the presence of poliovirus and in the production of antibodies against poliovirus. It has also been found that full-length poliovirus cDNA is infectious, which means it can be employed in producing altered virus particles for vaccines.
REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
Luria, General Virology, 1976, Wily & Sons, NY, 3rd Ed., pp. 312-315.
Kitamura, et al., Sequence of 1060 3'-terminal nucleotides of polio virus RNA a determined by a modification of the dideozynucleotide method, Proc. Natl. Acad. Sci., vol. 77, 1980, pp. 3196-3200.
Watson, Molecular Biology of the Gene, 1977, Benjamin Inc., London, 1977, pp. 655-656 and 591-592.
Nature, vol. 289, No. 5798, Feb. 12, 1981, pp. 555-559, H. Kupper et al.: "Cloning of cDNA of major antigen of foot and mouth disease virus and expression in E. coli".
Science, vol. 214, Nov. 20, 1981, pp. 916-919, V. R. Racaniello et al.: "Cloned Poliovirus Complementary DNA Is Infectious In Mammalian Cells".
Proc. Natl. Acad. Sci. USA, vol. 73, No. 7, Jul. 1976, D. L. Kacian et al.: "Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities".
Porter et al. (1978), Nature (London), 276, 298-301.
Taylor et al. (1976), Biochem. Biophys. Acta 442, 324-330.
Racaniello et al. (1981). Proc. Nat'l Acad. Sci. USA 78(g), 4887-4891.
Baltimore David
Racaniello Vincent R.
Massachusetts Institute of Technology
Nucker Christine M.
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