Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1993-08-19
1996-04-09
Jones, W. Gary
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 551, 536 2532, 536 231, C07H 504, C07H 506
Patent
active
055063500
ABSTRACT:
Techniques for producing cloned DNA sequences are provided which sequences are complementary to DNA occurring in one selected region of one chromosome of a multi-chromosomal genome, such as the human genome. Such cloned DNA sequences can be labeled and formed into probes by conventional procedures, there are provided methods for making probe compositions which comprise mixed DNA segments derived from such a DNA sequence. An improved DNA sequence transamination procedure is provided utilizing trifluoroacetate chaotrope anions. With high concentrations of low complexity DNA, high levels of transamination are thereby achieved. These segments are covalently bound to fluorophore groups through linking groups that are transaminated preferably chaotropically into the segments.
REFERENCES:
Draper, Nucleic Acid Res 12:989-1002 (1984) "Attachment of Reporter groups to specific . . . ".
Bittner Michael L.
Cruickshank Kenneth A.
Prorok Clarissa F.
Stols Lucy M.
Campbell Eggerton
Jones W. Gary
Murray William E.
LandOfFree
Production of chromosome region specific DNA sequences and trans does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Production of chromosome region specific DNA sequences and trans, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of chromosome region specific DNA sequences and trans will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-140205