Production of cholesterol esterase using Nocardia cholesterolicu

Details Production of cholesterol esterase using Nocardia cholesterolicu Production of cholesterol esterase using Nocardia cholesterolicu

1975-12-11

1977-10-04

Jones, Raymond N.

Textiles: fiber preparation

195 62, 195 65, 195114, C12D 1300, C07G 702, C12B 120

Patent

active

040522636

ABSTRACT:
An improved fermentation method comprises growing Nocardia cholesterolicum in a medium comprising a carbon source, such as glycerol, yeast extract, a nonionic surfactant, an auxiliary carbon source which is also an inducer of cholesterol oxidase, and trace salts. Yields of cholesterol oxidase up to about 900 U. (international units) per liter have been obtained using the improved method of the present invention in contrast with the yields of 1-3 U. per liter obtained in the prior art. Proper selection of environmental conditions also enables the coproduction of cholesterol esterase.

REFERENCES:
patent: 3776816 (1973-12-01), Terada et al.
patent: 3884764 (1975-05-01), Goodhue et al.
patent: 3909359 (1975-09-01), Goodhue et al.
Lee, "Degradation Mechanism of Cholesterol and its Derivatives by Microorganisms III," Chemical Abstracts, vol. 77, No. 23, p. 58, Abs. No. 147941f (1972).
Voets et al., "Microbial Degradation of Cholesterol," Chemical Abstracts, vol. 81, No. 1, p. 96, Abs. No. 1140e (1974).
Reese et al., "Surfactants as Stimulants of Enzyme Production by Microorganisms," Applied Microbiology, vol. 17, No. 2 (1969), pp. 242-245.
Research Disclosure, (Oct. 1974), "Method for the Preparation of Cholesterol Oxidase," pp. 46-50.
Sih et al., "An Efficient Synthesis of Estrone and 19-Norsteroids from Cholesterol," Journal of American Chemical Society, vol. 87, No. 12 (1965), pp. 2765-2766.
Korzenousky et al., "Manometric Assay and Properties of Pancreatic Esterase," Biochem. Journal, vol. 76 (1960), pp. 238-245.

Affiliated with

Also associated with

Rating

Production of cholesterol esterase using Nocardia cholesterolicu does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Production of cholesterol esterase using Nocardia cholesterolicu, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of cholesterol esterase using Nocardia cholesterolicu will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1773243