Production of chimeric bovine or porcine animals using cultured

Details Production of chimeric bovine or porcine animals using cultured Production of chimeric bovine or porcine animals using cultured

1996-12-16

1999-11-30

Campell, Bruce R.

Multicellular living organisms and unmodified parts thereof and

Method of making a transgenic nonhuman animal

800 15, 800 17, 435373, 435325, 435378, 435379, 435380, 435381, 435455, C12N 1509, C12N 1500, C12N 500, C12N 1563

Patent

active

059946199

ABSTRACT:
Novel cultured inner cell mass (CICM) cells, and cell lines, derived from ungulates, in particular, pigs and cows, and methods for their preparation are provided. The subject CICMs possess similar morphology and express cell markers identically or substantially similarly to ICMs of undifferentiated developing embryos for prolonged culturing periods. Heterologous DNA is inserted into the subject CICM cells and cell lines so as produce transgenic CICM cell which are introduced into non-human fertilized embryos to produce transgenic chimeric embryos. The transgenic chimeric embryos are transferred into recipient females where they are permitted to develop into transgenic chimeric fetuses. Recipient females give birth to transgenic chimeric animals which are capable of transmitting the heterologous DNA to their progeny. Transgenic CICM cells are also used to produce cloned transgenic embryos, fetuses and offspring.

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