Production of cartilage tissue using cells isolated from Wharton

Details Production of cartilage tissue using cells isolated from Wharton Production of cartilage tissue using cells isolated from Wharton

1996-10-23

1999-07-06

Stanton, Brian R.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of detaching cells, digesting tissue or establishing...

435366, 435377, 435325, 424 931, C12N 500, C12N 502, A01N 6300

Patent

active

059197021

ABSTRACT:
The invention relates to the isolation and use of pre-chondrocytes from the umbilical cord, specifically from Wharton's jelly, that give rise to chondrocytes which produce cartilage. The isolated pre-chondrocytes, or the chondrocytes to which they give rise, can be mitotically expanded in culture and used in the production of new cartilage tissue for therapeutic use. "Banks" of pre-chondrocytes or chondrocytes can be stored frozen, and thawed and used to produce new cartilage tissue as needed.

REFERENCES:
patent: 4352883 (1982-10-01), Lim
patent: 4609551 (1986-09-01), Caplan et al.
patent: 4846835 (1989-07-01), Grande
patent: 4963489 (1990-10-01), Naughton et al.
patent: 5041138 (1991-08-01), Vacanti et al.
patent: 5197985 (1993-03-01), Caplan et al.
patent: 5226914 (1993-07-01), Caplan et al.
patent: 5486359 (1996-01-01), Caplan et al.
Sandell et al. Alternatively spliced type II procollagen mRNAs define distinct populations of cells during vertebral development: differential expression of the amino-propeptide. Journal of Cell Biology, vol. 114, No. 6, pp. 1307-1319, Sep. 1991.
Elima et al. Expression of mRNAs for collagens and other matrix components in dedifferentiating and redifferentiating human chondrocytes in culture. FEBS letters, vol. 258, No. 2, pp. 195-198, 1989.
Grande, D.A. et al., 1995, "Repair of Articular Cartilage Defects Using Mesenchymal Stem Cells", Tissue Engineering 1: 345-353.
Corvetti and Cameron-Curry, 1986, "Immunohistochemical and Ultrastructural Study of Two Human Loose Mucoid Connective Tissues", Arch. Ital. Anat. Embriol. 91(1):9-19 and Biol. Abstr. 83(5):111 (Abstr. No. 40785, 1987).
Naughton et al., 1997, "Cells Isolated from Wharton's Jelly of the Human Umbilical Cord Develop a Cartilage Phenotype when Treated with TGF.sub..beta. In Vitro", FASEB J. 11(3):A19 (Abstr. No. 108).
Adams, J. et al., 1995, "The c-myc Oncogene Driven by Immunboglobulin Enhancers Inuces Lymphoid Malignancy in Transgenic Mice", Nature 318:533-538.
Alexander et al., 1987, "Expression of the c-myc Oncogene under Control of an Immunoglobulin Enhancer in E.mu.-myc Transgenic Mice", Mol. Cell Biol. 7:1436-1444.
Alexandrow and Moses, 1995, "Transforming Growth Factor .beta. and Cell Cycle Regulation", Cancer Res. 55:1452-1457. phosphazenes!.sup.1-3 ", Macromolecules 10:824-830.
Assoian, R. et al., 1983, "Transforming Growth Factor-.beta. in Human Platelets", J. of Biol. Chem. 258:7155-7160.
Barnard, J.et al., 1990, "The Cell Biology of Transforming Growth Factor .beta.", Biochem. Biophys. Acta 1032:79-87.
Beck, et al., 1991, "In Vivo Induction of Bone by Recombinant Human Transforming Growth Factor .beta..sub.1 ", J. Bone Mineral Res. 6:961-968.
Burch, W. et al., 1985, "Homologous and Heterologous Growth Hormones Fail to Stimulate Avian Cartilage Growth in Vitro", J. Clin. Endocrinol. Metab. 60:747-750.
Campbell, I. et al., 1991, "Human Articular Cartilage and Chondrocytes Produce Hemopoietic Colony-Stimulating Factors in Culture in Response to IL-1", J. Immunology 147:1238-1246.
Caplan, A., 1991, "Mesenchymal Stem Cells", J. of Orthopaedic Res. 9:641-650.
Cate, R. et al., 1986, "Isolation of the Bovine and Human Genes for Mullerian Inhibiting Substance and Expression of the Human Gene in Animal Cells", Cell 45:685-698.
Centrella, M. et al., 1987, "Transforming Growth Factor .beta. is a Bifunctional Regulator of Replication and Collagen Synthesis in Osteoblast-enriched Cell Cultures from Fetal Rat Bone", J. Biol. Chem. 262:2869-2874.
Coffey, R. et al., 1988, "Growth Modulation of Mouse Keratinocytes by Transforming Growth Factors", Cancer Res. 48:1596-1602.
Cormack, D., 1987, "Ham's Histology", J.B. Lippincott Company, pp. 266-272.
DeMartin et al., 1987, "Complementary DNA for Human Glioblastoma-derived T Cell Suppressor Factor, a Novel Member of the Transforming Growth Factor-.beta. Gene Family", EMBO J. 6:3676-3677.
Derynck, R. et al., 1988, "A New Type of Transforming Growth Factor-.beta.TGF-.beta.3", EMBO J. 7:3737-3743.
Derynck, R. et al., 1985, "Human Transforming Growth Factor-.beta. Complementary DNA Sequence and Expression in Normal and Transformed Cells", Nature 316:701-705.
Domb, A., et al., 1992, "Degradable Polymers for Site Specific Drug Delivery," 3:279-292.
Freshney, R.I., 1987, "Culture of Animal Cells", Alan R. Liss, Inc., New York, Chapt. 14, p. 187.
Galera, P. et al., 1992, "Transforming Growth Factor-.beta.1 (TGF-.beta.1) Up-Regulation of Collagen Type II in Primary Cultures of Rabbit Articular Chondrocytes (RAC) Involves Increased mRNA Levels without Affecting mRNA Stability and Procollagen Processing", J. Cell Physio. 153:596-606.
Gentry, L. et al., 1987, "Type 1 Transforming Growth Factor Beta: Amplified Expression and Secretion of Mature and Precursor Polypeptides in Chinese Hamster Ovary Cells", Mol. and Cell. Biol. 7:3418-3427.
Gill, P. et al., 1993, "Wharton's Jelly in the Umbilical Cord", J. of Reprod. Med. 38:611-614.
Goey, H. et al., 1989, "Inhibition of Early Murine Hemopoietic Progenitor Cell Proliferation after In Vivo Locoregional Administration of Transforming Growth Factor-.beta.1", J. of Immunol. 143:877-880.
Goldkorn et al., 1992, "Transforming Growth Factor .beta. Modulates Phosphorylation of the Epidermal Growth Factor Receptor and Proliferation of A431 Cells," Cell Growth and Diff. 3:101-109.
Goldring et al., 1988, "Interleukin 1 Suppresses Expression of Cartilage-specific Types II and IX Collagens and Increases Types I and III Collagens in Human Chondrocytes", J. Clin. Invest. 82:2026-2037.
Grosschedl, R. et al., 1984, "Introduction of a .mu. Immunoglobulin Gene into the Mouse Germ Line: Specific Expression in Lymphoid Cells and Synthesis of Functonal Antibody", Cell 38:647-658.
Hamerman, D. et al., 1986, "A Cartilage-Derived Growth Factor Enhances Hyaluronate Synthesis and Diminishes Sulfated Glycosaminoglycan Synthesis in Chondrocytes", J. Cell. Physio. 127:317-322.
Hanahan, D., 1985, "Heritable Formation of Pancreatic .beta.-Cell Tumors in Transgenic Mice Expressing Recombinant Insulin/Simian Virus 40 Oncogenes", Nature 315:115-122.
Hanks, S. et al., 1988, "Amino Acid Sequence of the BSC-1 Cell Growth Inhibitor (Polyergin) Deduced from the Nucleotide Sequence of the cDNA", PNAS USA 85:79-82.
Hattersley et al., 1995, J. Bone and Mineral Res. 10:S163.
Horton, W. et al., 1989, "Transforming Growth Factor-Beta and Fibroblast Growth Factor Act Synergistically to Inhibit Collagen II Synthesis through a Mechanism Involving Regulatory DNA Sequences", J. Cell. Physio. 141:8-15.
Ignotz and Massague, 1986, "Transforming Growth Factor-.beta. Stimulates the Expression of Fibronectin and Collagen and Their Incorporation into the Extracellular Matrix", J. Biol. Chem. 26:4337-4345.
Ikebe, T. et al., 1988, "Effects of Human Recombinant Tumor Necrosis Factor-.alpha. and Interleukin 1 on the Synthesis of Glycosaminoglycan and DNA in Cultured Rat Costal Chondrocytes", J. Immunol. 140:827-831.
Ikeda, T. et al., 1987, "Human transforming Growth Factor Type .beta.2: Production by a Prostatic Adenocarcinoma Cell Line, Purification, and Initial Characterization", Biochemistry 26:2406-2410.
Inada, M. et al., 1996, "Bone Morphogenetic Protein 12 and -13 Inhibit Terminal Differentiation of Myoblasts, but Do Not Induce Their Differentiation into Osteoblasts", Biochem. and Biophys. Res. Comm. 222:317-322.
Jackson, R.W., 1983, "Current Concepts Review Arthroscopic Surgery", J. of Bone and Joint Surgery 65-A:416-420.
Jakowlew et al., 1988, "Complementary Deoxyribonucleic Acid Cloning of a Novel Transforming Growth Factor-.beta. Messenger Ribonucleic Acid from Chick Embryo Chondrocytes", Mol. Endocrinol. 2:747-755.
Jakowlew et al., 1988, "A Novel Low Molecular Weight Ribonucleic Acid (RNA) Related to Transforming Growth Factor .alpha. Messenger RNA", Mol. Endocrinol. 2:1056-1063.
Joyce, M. et al., 1990, "Transforming Growth Factor-.beta. and the Initiation of Chondrogenesis and Osteogenesis in the Rat Femur", J. Cell Biol. 110:2195-2207.
Kasid, A. et al., 1988, "Effects of Transforming Growth Factor-.beta. on Human Lymphokine-activated Killer Cell Precursors",

Affiliated with

Also associated with

Rating

Production of cartilage tissue using cells isolated from Wharton does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Production of cartilage tissue using cells isolated from Wharton, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of cartilage tissue using cells isolated from Wharton will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-898738