Production of biologically active recombinant insulin-like growt

Details Production of biologically active recombinant insulin-like growt Production of biologically active recombinant insulin-like growt

1998-01-07

2000-01-04

Ulm, John

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 435325, 435243, 4353201, 536 231, 536 2351, 530399, C12N 1518, C12N 1563, C12N 500, C07K 1465

Patent

active

060108827

ABSTRACT:
The present invention relates to the finding and construction of fish insulin-like growth factor II (IGF-II) cDNAs which can be cloned and expressed in cells. This invention also relates to the production of biologically active fish IGF-II polypeptides by a gene expression system using fish IGF-II cDNAs. The fish IGF-II cDNAs have 1977 bp which transcribe into a prepeptide (signal peptide), and B, C, A, D, E domain peptides. The fish mature IGF-II is a single polypeptide containing the NH.sub.2 -B-C-A-D-COOH domains. The mature IGF-II polypeptide is 7 kDa in weight and has 70 amino acids. The fish recombinant IGF-II CDNA can be cloned and expressed in E. coli, yeast, baculovirus, and fish cells.

REFERENCES:
patent: 5476779 (1995-12-01), Chen et al.
Rentui-Delrue et al. DNA 8(4):261-270, 1989.
Rentui-Delrue et al. DNA 8(4):271-278, 1989.
DNA and Cell Biology, J.Y. Chen et al., Production of Biologically Active Recombinant Tilapia Insulin-Like Growth Factor-II Polypeptides in Escherichia coli Cell and Characterization of the Genomic Structure of the Coding Region, 16:-883-892 (T) 1997.
DNA, Alane Gray et al., Tissue-Specific and Developmentally Regulated Transcription of the Insulin-Like Growth Factor 2 Gene, vol. 6, No. 4, 1987.
Histochemistry, Reinecke et al., IGF-2-like peptides are present in insulin cells of the elasmobranchian endocrine pancreas: an immunohistochemical and chromatographic study, 102:365-371 (1994).
Proc. Natl. Acad. Sci. USA, Michael Shamblott, Identification of a second insulin-like growth factor in a fish species, 89:8913-8917 1992.
DNA and Cell Biology, Vadim Kavsan, Structure of the Chum Salmon Insulin-Like Growth Factor I Gene, 12:729-737 1993.

Affiliated with

Also associated with

Rating

Production of biologically active recombinant insulin-like growt does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Production of biologically active recombinant insulin-like growt, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of biologically active recombinant insulin-like growt will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1071850