Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1989-08-25
1992-10-27
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
530303, 530305, 530324, 530399, 435 694, 435 697, C12P 2102, C12P 2106, C07K 740, C07K 710
Patent
active
051588757
ABSTRACT:
A novel method for refolding reduced IGF-I is provided in accordance with the present invention. The method is carried out by providing additional positive charge at the amino terminal end of the IGF-I molecule by the presence of a short leader sequence. The additional positive charge on the met-end of the IGF-I molecule has been found to enable recombinant IGF-I to refold simply by stirring solubilized inclusion body protein for between 2-16 hours, or overnight. The present invention also provides a novel fusion protein intermediate of IGF-I attached to the positively charged leader sequence.
REFERENCES:
patent: 4532207 (1985-07-01), Brewer et al.
Peters, M. A. 1985, "Expression of a biologically active analogue of somatomedin-c/insulin-like growth factor I", Gene, vol. 35, pp. 83-89.
Hsieh Philip K.
Miller James A.
Tsai Larry B.
Abers Julia E.
Amgen Inc.
Mosher Mary E.
Schwartz Richard A.
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