Multicellular living organisms and unmodified parts thereof and – Method of using a transgenic nonhuman animal to manufacture... – The protein is isolated or extracted from milk
Reexamination Certificate
2007-01-02
2007-01-02
Falk, Anne-Marie (Department: 1632)
Multicellular living organisms and unmodified parts thereof and
Method of using a transgenic nonhuman animal to manufacture...
The protein is isolated or extracted from milk
C800S004000, C800S013000, C800S014000, C800S015000, C800S016000
Reexamination Certificate
active
09040518
ABSTRACT:
Disclosed is a method for the recombinant production of biofilaments, such as spider silk or insect fibroins, using transgenic animals which secrete the biofilaments in their milk and/or urine, and transgenic cells which secrete the biofilaments into culture media. Such a method is useful for producing large quantities of biofilament material. Also disclosed is a nucleic acid molecule for generating such transgenic animals.
REFERENCES:
patent: 4423145 (1983-12-01), Stampfer et al.
patent: 4873316 (1989-10-01), Meade et al.
patent: 5227301 (1993-07-01), Turner et al.
patent: 5243038 (1993-09-01), Ferrari et al.
patent: 5304489 (1994-04-01), Rosen
patent: 0 771 874 (1997-07-01), None
patent: WO88/01648 (1988-03-01), None
patent: WO91/08216 (1991-06-01), None
patent: WO93/25567 (1993-12-01), None
patent: WO96/39494 (1996-12-01), None
patent: WO 97/08315 (1997-06-01), None
Bradley et al. Modifying the mouse: Design and desire. Bio/Technology 10: 534-539, May 1992.
Campbell and Wilmut, Totipotency and multipotentiality of cultured cells: Applications and progress. Theriogenology 47: 63-72, Jan. 1997.
Wall, R.J. Transgenic livestock: Progress and prospects for the future. Theriogenology 45: 57-68, May 1992.
Hayashi et al., “Evidence from Flagelliform Silk cDNA for the Structural Basis of Elasticity and Modular Nature of Spider Silks,” Journal of Molecular Biology 275:773-784 (1998).
Kerr et al., “The Bladder as a Bioreactor: Urothelium Production and Secretion of Growth Hormone into Urine,” Nature Biotechnology 16:75-79 (1998).
Meyer-Puttlitz et al., “Ectopic Expression of a BacteriallacZGene in the Limbic System of Transgenic Mice,” NeuroReport 6:1674-1678 (1995).
Lee et al., “Production of Biomedical Proteins in the Milk of Transgenic Dairy Cows: the State of the Art,” Journal of Controlled Release 29:213-221 (1994).
Parkhe et al., “Structural Studies of Spider Silk Proteins in the Fiber,” Journal of Molecular Recognition 10:1-6 (1997).
Prince et al., “Construction, Cloning, and Expression of Synthetic Genes Encoding Spider Dragline Silk,” Biochemistry 34:10879-10885 (1995).
Romagnolo et al., “Transgenic Approaches for Modifying the Mammary Gland to Produce Therapeutic Proteins,” Environmental Health Perspectives 102:846-851 (1994).
Guerette et al., “Silk Properties Determined by Gland-Specific Expression of a Spider Fibroin Gene Family,” Science 272: 112-115, 1996.
Arcidiacono et al., “Purification and characterization of Recombinant Spider Expressed inEscherichia coli,” Appl. Microbiol. Biotechnol. 49: 31-38, 1998.
Gosline et al., “The Macromolecular Design of Spiders' Silks,” Biomimetics: Design and Processing of Materials, pp. 237-261, eds. Sarikaya and Aksay, Amer. Inst. of Physics, 1995.
Xu and Lewis, “Structure of a Protein Superfiber: Spider Dragline silk,” Proc. Natl. Acad. Sci. U.S.A. 87: 7120-7124, 1990.
Beckwitt and Arcidiacono, “Sequence Conservation in the C-Terminal Region of Spider Silk Protein (Spidroin) fromNephila clavipes(Tetragnathidae) andAraneus bicentenarius(Araneidae),” J. Biol. Chem. 269: 6661-6663, 1994.
Fahnestock and Irwin, “Synthetic Spider Dragline Silk Proteins and their Production inEscherichia coli,” Appl. Microbiol. Biotechnol. 47: 23-32, 1997.
Lee et al., “Tissue-Specific Expression of the rat β -Casein Gene in Transgenic Mice,” Nucleic Acids Res. 16: 1027-1041, 1988.
Vilotte et al., “Efficient Tissue-Specific Expression of Bovine α-Lactalbumin in Transgenic Mice,” Eur. J. Biochem. 186: 43-48, 1989.
Tsujimoto and Suzuki, “The DNA Sequence of Bombyx mori Fibroin Gene Including the 5′ Flanking, mRNA Coding, Entire Intervening and Fibroin Protein Coding Regions,” Cell 18: 591-600, 1979.
Tsujimoto and Suzuki, “Structural Analysis of the Fibroin Gene at the 5′ End and its Surrounding Regions,” Cell 16: 425-436, 1979.
Huynh et al., “Establishment of Bovine Mammary Epithelial Cells (MAC-T): An in Vitro Model for Bovine Lactation,” Exp. Cell. Res. 197: 191-199, 1991.
Ichimura and Mita, “Essential Role of Duplications of Short Motif Sequences in the Genomic Evolution toBombyx mori,” J. Mol. Evol. 35: 123-130, 1992.
Ebert et al., “Induction of Human Tissue Plasminogen Activator in the Mammary Gland of Transgenic Goats,” Bio/Technology 12: 699-701, 1994.
Velander et al., “High-level Expression of a Heterologous Protein in the Milk of Transgenic Swine Using the cDNA Encoding Human Protein C,” Proc. Natl. Acad. Sci. U.S.A. 89: 12003-12007, 1992.
Velander et al., “Production of Biologically Active Human Protein C in the Milk of Transgenic Mice,” Ann. N. Y. Acad Sci. 665: 391-403, 1992.
Campbell et al., “Comparison of the Whey Acidic Protein Genes of the Rat and Mouse,” Nucleic Acids Res. 12: 8685-8697, 1984.
Hennighausen et al., “Comprarative Sequence Analysis of the mRNAs Coding for Mouse and Rat Whey Protein,” Nucl. Acids Res. 10: 3733-3744, 1982.
Lin et al., “A Tissue-Specific Promoter that Can Drive a Foreign Gene to Express in the Suprabasal Urothelial Cells of Transgenic Mice” Proc. Natl. Acad. Sci. U.S.A. 92:679-683, 1995.
Peschon et al., “Supermatid-Specific Expression of Protamine 1 in Transgenic Mice,” Proc. Natl. Acad. Sci. U.S.A. 84: 5316-5319, 1987.
Groenen and Van der Poel, “Regulation of Expression of Milk Protein Genes: A Review,” Livestock Production Science 38: 61-78, 1994.
Karatzas Costas N.
Lazaris-Karatzas Anthoula
Turner Jeffrey D.
Falk Anne-Marie
Jones Day
Nexia Biotechnologies Inc.
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