Production of beta-hydroxypalmitate methyl esterase from...

Details Production of beta-hydroxypalmitate methyl esterase from... Production of beta-hydroxypalmitate methyl esterase from...

2004-03-16

2008-03-11

Hobbs, Lisa J (Department: 1657)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S134000, C435S252100

Reexamination Certificate

active

07341857

ABSTRACT:
The present invention provides a novel esterase derived fromIdeonellasp. 0-0013 strain (FERM BP-08660) having the following properties: (1) function, substrate specificity: hydrolyzes methyl 3-hydroxypalmitate to generate 3-hydroxypalmitic acid and methanol; (2) optimal temperature for functioning: 37° C.; (3) optimal pH and stable pH range: pH 7 or more to pH 10 or less; (4) temperature stability: 97% of the enzyme is stable at 43° C.; (5) inhibition, activation, and stabilization: activated by sodium ion and potassium ion, and inhibited by strontium ion, iron ion (divalent), and methyl palmitate; (6) molecular weight: about 46,500 Da (by SDS-PAGE), about 41,000 Da (by a gel filtration method); and (7) isoelectric point: pI 4 (by polyacrylamide gel isoelectric focusing method); a microorganism producing the enzyme; and a method of producing the enzyme.

REFERENCES:
patent: 11-56349 (1999-03-01), None
patent: 11-56364 (1999-03-01), None
Shinohara et al. (2007) Purification and characterization of a novel esterase (B-hydroxypalmitate methyl ester hydrolase) and prevention of the expression of virulence by Ralstonia solanacearum. J. Appl. Microbiol. 103:152-162.
Macrae, A. (2000) The Use of 16S rDNA Methods is Soil Microbial Ecology. Brazilian J. Microbiol. 31:77-82.

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