Production of aryl acylamidases

Details Production of aryl acylamidases Production of aryl acylamidases

1981-12-01

1984-02-07

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435253, 435815, 435876, 435877, C12N 980, C12N 120, C12R 139, C12R 140

Patent

active

044304331

ABSTRACT:
A process for the production of an aryl acylamidase enzyme involves culturing bacteria of one of the strains Pseudomonas fluorescens ATCC 39005 (or suitable mutants or variants thereof) or Pseudomonas putida ATCC 39004 (or suitable mutants or variants thereof) in a culture medium in which the bacterial strains produce aryl acylamidase and collecting the enzyme containing material, generally the cell material. Preferably the resulting cells are then disrupted, especially by enzymatic treatment, and the aryl acylamidase is separated from the other unwanted substances, generally the other cell constituents.
Preferably the culture medium contains N-acylaniline, especially N-acetyl aniline. The N-acylaniline may form part of a complex or defined salts medium.

REFERENCES:
Engelhardt et al., Applied Microbiology, vol. 26, No. 5, pp. 709-718 (1973).
Hsiung et al., Biochemical and Biophysical Research Communications, vol. 66, No. 4, pp. 1225-1230 (1975).

Affiliated with

Also associated with

Rating

Production of aryl acylamidases does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Production of aryl acylamidases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of aryl acylamidases will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2162254