Multicellular living organisms and unmodified parts thereof and – Nonhuman animal – Transgenic nonhuman animal
Reexamination Certificate
2006-12-05
2006-12-05
Wehbé, Anne Marie S. (Department: 1633)
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
Transgenic nonhuman animal
C800S013000, C800S006000, C800S021000, C435S325000, C435S320100, C435S455000, C435S326000, C435S462000
Reexamination Certificate
active
07145056
ABSTRACT:
A method to produce a cell expressing an antibody from a genomic sequence of the cell comprising a modified immunoglobulin locus using Cre-mediated site-specific recombination is disclosed. The method involves first transfecting an antibody-producing cell with a homology-targeting vector comprising a lox site and a targeting sequence homologous to a first DNA sequence adjacent to the region of the immunoglobulin loci of the genomic sequence which is to be converted to a modified region, so the first lox site is inserted into the genomic sequence via site-specific homologous recombination. Then the cell is transfected with a lox-targeting vector comprising a second lox site suitable for Cre-mediated recombination with the integrated lox site and a modifying sequence to convert the region of the immunoglobulin loci to the modified region. This conversion is performed by interacting the lox sites with Cre in vivo, so that the modifying sequence inserts into the genomic sequence via Cre-mediated site-specific recombination of the lox sites. Also disclosed are a form of the method used to produce a cell expressing a modified antibody molecule using Cre-mediated site-specific recombination, and antibody-producing cells obtainable by the disclosed methods. Class-switching modifications of human antibodies produced in murine hybridoma cells are exemplified.
REFERENCES:
patent: 4816567 (1989-03-01), Cabilly et al.
patent: 4959317 (1990-09-01), Sauer
patent: 5202238 (1993-04-01), Fell et al.
patent: 5434066 (1995-07-01), Bebee et al.
patent: 5434340 (1995-07-01), Krimpenfort et al.
patent: 5545806 (1996-08-01), Lonberg et al.
patent: 6091001 (2000-07-01), Jakobovits et al.
patent: 6130364 (2000-10-01), Jakobovits et al.
patent: 6458592 (2002-10-01), Jakobovits et al.
patent: 0314 161 (1989-05-01), None
patent: WO 86/01533 (1986-03-01), None
patent: WO 91/10741 (1991-07-01), None
patent: WO 93/12227 (1993-06-01), None
patent: WO 93/19172 (1993-09-01), None
patent: WO 94/02602 (1994-02-01), None
patent: WO 94/13804 (1994-06-01), None
patent: WO 94/04667 (2004-03-01), None
Campbell et al. (1997) Theriology. vol. 47 (1), 65-72.
Deonarain (1998) Exp. Opin. Ther. Patents. vol. 8(1) 53-69.
Zou et al. (1994) Current Biology. vol. 4(12), 1099-1103.
Miller et al. (1995) FASEB. vol. 9, 190-199.
Alvarado-Urbina et al. (1981). “Automated synthesis of gene fragments”Science, vol. 214: 270-274.
Blochlinger et al. (1984). “hygromycin B Phosphotransferase as a selectable marker for DNA transfer expression with higher eucaryotic cells”Mol. Cell. Biol., vol. 4: 2929-2931.
Campbell et al. (1997).Theriology, vol. 47(1):65.
Capecchi (1989). “Altering the genome by homologous recombination”Science, vol. 244: 1288-1292.
Graham et al. (1973). “A new technique for the assay of infectivity of human adenovirus 5 DNA”Virology, vol. 52.
Gorman et al. (1982). “The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into eukaryotic cells by DNA-mediated transfection”Proc. Natl. Acad. Sci. USA, vol. 79: 6777-6781.
Gu et al. (1993). “Independent control of immunoglobuli8n switch recombination at individual switch regions expression through Cre-IoxP-mediated gene targeting”Cell, vol. 73: 1155-1164.
Hoess et al. (1982). “P1 site-specific recombination: nucleotide sequence of the recombining sites”Proc. Natl. Acad. Sci. USA, vol. 79:3398-3402.
Ito et al. (1982). “Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers solid support”Nuc. Acids Res., vol. 10: 1755-1769.
Kohler et al. (1975). vol. 256: 495-497.
Miller et al. (1995).FASEB, vol. 9: 190-199.
Neuberger et al. (1984). “Recombinant antibodies possessing novel effector functions”Nature, vol. 312: 604-608.
Pavlakis et al. (1983). “Regulation of a metallothionein-growth hormone hybrid gene in bovine papilloma virus”Proc. Natl. Acad. Sci. USA, vol. 80: 397-401.
Southern et al. (1982). “Transformation of mammalian cells to antibiotic resistance with a bacterial gene under the SV40 early region promoter”J. Mol. Appl. Genet., vol. 1: 327-341.
Spira et al. (1984). The identification of monoclonal class switch variants by sib selection and an ELISAImmunological Methods, vol. 74: 307-315.
Steplewski et al. (1985).“Isolation and characterization of anti-monodialoganglioside monoclonal antibody 19 switch variants”Proc. Natl. Acad. Sci. USA, vol. 82: 8653-8657.
Tan et al. (1985). “A human-mouse chimeric immunoglobulin gene with a human variable region is expressed in cells”J. Immunol., vol. 135: 3564-3567.
Waterhouse et al. “Combinatorial infection andin vivorecombination: a strategy for making large phase repertiores”Nucleic Acids Res., vol. 21: 2265-2266.
Zou et al. (1994).Current Biology, vol. 4(12): 1099-1103.
Smith et al. “A site-directed chromosomal translocation induced in embryonic stem cells by cre-loxp recombination recombination” Nature Genetics, NY, NY, vol. 9 Apr. 1, 1995, pp. 376-385.
Jakobovits Aya
Zsebo Krisztina M.
Abgenix, Inc.
Borden Paula A.
Bozicevic Field & Francis LLP
Francis Carol L.
Japan Tobacco Inc.
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