Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1979-02-22
1980-11-18
Castel, Benoit
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
210610, 210925, 435281, C12P 1904
Patent
active
042346894
ABSTRACT:
Growth of Arthrobacter Sp. ATCC 31012 on ethanol has been used to produce a new class of extracellular micro bial protein-associated lipopolysaccharides (the ".alpha.-emulsans") which, on a weight-for-weight basis, are probably the most efficient emulsifiers discovered and which possess certain characteristics that permit these unique extracellular microbial lipopolysaccharides to be widely used in cleaning oil-contaminated vessels, oil spill management, and enhanced oil recovery by chemical flooding. Deproteinization of .alpha.-emulsans by hot phenol extraction produces the lipopolysaccharide components (the "apo-.alpha.-emulsans") of such .alpha.-emulsans, which components have been shown to be completely N-acylated and partially O-acylated heteropolysaccharides made up of major amounts of D-galactosamine and an aminouronic acid, the O-lipoacyl portions of such apo-.alpha.-emulsans containing at least 5 percent by weight of fatty acid esters in which the fatty acids contain from about 10 to about 18 carbon atoms. .alpha.-Emulsans and apo-.alpha.-emulsans, both of which biopolymers are strongly anionic, exhibit a high degree of specificity in the emulsification of hydrocarbon substrates which contain both aliphatic and cyclic components. In addition, these extracellular microbial polysaccharides as well as their O-deacylated and N-deacylated derivatives are adsorbed on and capable of flocculating aluminosilicate ion-exchangers, such as kaolin and bentonite.
REFERENCES:
patent: 3941692 (1976-03-01), Gutnick et al.
patent: 3997398 (1976-12-01), Zajic et al.
Reisfeld, A., et al.; "Applied Microbiology," 24, 363 (1972).
Englander, E., et al.; "Int. Assoc. Microbiol. Soc. Abstracts," vol. II, p. 201 (1973).
Rosenberg, E., et al.; "EPA Report 600-3-75-001"; pp. 157-168 (Dec. 1974).
Horowitz, A., et al.; "Applied Microbiology," 30, 10 (1975).
Gutnick David L.
Rosenberg Eugene
Shabtai Yossef
Biotechnologie Aktiengesellschaft fur Emulsan
Castel Benoit
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