Production of .alpha.-emulsans

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435281, 210611, 210925, C12P 1904

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active

042308011

ABSTRACT:
Growth of Arthrobacter Sp. ATCC 31012 on fatty acid substrates produces a new class of extracellular microbial protein-associated lipopolysaccharides (the ".alpha.-emulsans"). Deproteinization of .alpha.-emulsans by hot phenol extraction produces the lipopolysaccharide components (the "apo-.alpha.-emulsans") of such emulsans, which components have been shown to be completely N-acylated and partially O-acylated heteropolysaccharides made up of major amounts of D-galactosamine and an aminouronic acid, such apo-.alpha.-emulsans containing at least 5 percent by weight of O-substituted fatty acid esters in which the fatty acids contain from about 10 to about 18 carbon atoms. .alpha.-Emulsans and apo-.alpha.-emulsans, both of which biopolymers are strongly anionic, exhibit a high degree of specificity in the emulsification of hydrocarbon substrates which contain both aliphatic and cyclic components. In addition, these extracellular microbial polysaccharides as well as their O-deacylated and N-deacylated derivatives are adsorbed on and capable of flocculating aluminosilicate ion-exchangers, such as kaolin and bentonite.

REFERENCES:
patent: 3941692 (1976-03-01), Gutnick et al.
patent: 3997398 (1976-12-01), Zajic et al.
Reisfeld A. et al., "Applied Microbiology", 24, 363 (1972).
Englander E. et al., "Int. Assoc. Microbiol. Soc. Abstracts", vol. II, p. 201 (1973).
Rosenberg E. et al., "EPA Report 600-3-75-001", pp. 157-168 (Dec. 1974).
Horowitz A. et al., "Applied Microbiology", 30, 10 (1975).

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