Production of a transgenic avian by cytoplasmic injection

Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal

Reexamination Certificate

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C800S019000

Reexamination Certificate

active

07550650

ABSTRACT:
This invention provides methods for the stable introduction of heterologous coding sequences into the genome of a bird and expressing the coding sequences to produce desired proteins or to alter the phenotype of the bird. The present invention provides preferred methods for introducing a transgene into the cytoplasm of avian embryonic cells by cytoplasmic microinjection. The embryo then develops into a transgenic adult capable of expressing a heterologous protein and/or capable of generating a line of transgenic birds through breeding. Synthetic vectors and gene promoters useful in the methods are also provided by the present invention, as are transgenic birds that express heterologous protein and avian eggs containing heterologous protein.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4603112 (1986-07-01), Paoletti et al.
patent: 4722848 (1988-02-01), Paoletti et al.
patent: 4769330 (1988-09-01), Paoletti et al.
patent: 4997763 (1991-03-01), Hughes et al.
patent: 5011780 (1991-04-01), Perry
patent: 5162215 (1992-11-01), Bosselman et al.
patent: 5174993 (1992-12-01), Paoletti et al.
patent: 5175384 (1992-12-01), Krimpenfort et al.
patent: 5338683 (1994-08-01), Paoletti et al.
patent: 5340740 (1994-08-01), Petitte et al.
patent: 5494807 (1996-02-01), Paoletti et al.
patent: 5505941 (1996-04-01), Paoletti et al.
patent: 5580859 (1996-12-01), Felgner et al.
patent: 5589466 (1996-12-01), Felgner et al.
patent: 5591639 (1997-01-01), Bebbington
patent: 5656479 (1997-08-01), Petitte et al.
patent: 5731178 (1998-03-01), Sippel et al.
patent: 6027722 (2000-02-01), Hodgson
patent: 6423488 (2002-07-01), Harvey
patent: 6730822 (2004-05-01), Ivarie et al.
patent: 2002/0108132 (2002-08-01), Rapp
patent: 2002/0116732 (2002-08-01), Christmann
patent: 2002/0199214 (2002-12-01), Rapp
patent: 2003/0126628 (2003-07-01), Harvey et al.
patent: 2003/0140363 (2003-07-01), Rapp
patent: WO 87/05325 (1987-09-01), None
patent: WO 90/11355 (1990-10-01), None
patent: WO 92/06180 (1992-04-01), None
patent: WO 92/19749 (1992-11-01), None
patent: WO 92/20316 (1992-11-01), None
patent: WO 92/22635 (1992-12-01), None
patent: WO 93/04701 (1993-03-01), None
patent: WO 93/25234 (1993-12-01), None
patent: WO 94/06920 (1994-03-01), None
patent: WO 94/11524 (1994-05-01), None
patent: WO 97/47739 (1997-12-01), None
patent: WO 99/10505 (1999-03-01), None
patent: WO 99/19472 (1999-04-01), None
patent: WO 99/42569 (1999-08-01), None
patent: WO 00/09674 (2000-02-01), None
patent: WO 00/69257 (2000-11-01), None
patent: WO 0220752 (2002-03-01), None
patent: WO 02/064727 (2002-08-01), None
patent: WO 02/079447 (2002-10-01), None
Vick, Proc. R. Soc. Lond., 1993, vol. 251, p. 179-182.
Love, Bio/Technology, 1994, vol. 12, p. 60-63.
Tanaka (1994, J. Reprod. Fert., vol. 100, p. 447-449).
Thoraval, Transgenic Research, 1995, vol. 4, p. 369-376.
Sayegh, Dec. 15, 1999, vol. 72, p. 31-37.
Mohammed (1998, Immunotechnology, vol. 4, p. 115-125).
Ishida (2002, Cloning Stem Cells, vol. 4, p. 91-102) s.
Harvey (Nature Biotech, Apr. 2002, vol. 19, p. 396-399).
Ivarie (Trends in Biotechnology, Jan. 2003, vol. 21, p. 14-19).
Naito (Transgenic Animals: Generation and Use, 1997, Ed. By Louis Marie Houdebine, Harwood Academic Publishers, p. 69-73).
Bachiller et al. Liposome-mediated DNA uptake by sperm cells.Molecular Reproduction and Development. 1991, 30:194-200, Wiley-Liss, Inc.
Baldari et al., A novel leader peptide which allows efficient secretion of a fragment of human interleukin 1β inSaccharomyces cerevisiae E.M.B.O.J., 1987, 6:229-234.
Cibelli et al. Cloned transgenic calves produced from nonquiescent fetal fibroblasts.Science. May 22, 1998; 280:1256-1258.
Collas et al., Nuclear localization signals enhance germline transmission of a transgene in zebrafish , 1998,Transgenic Research7, 303-309.
Etches et al. Strategies for the production of transgenic chickens.Methods Mol Biol. 1997;62:433-50.
Eyestone and Campbell. Nuclear transfer from somatic cells: applications in farm animal species.J. Reprod Fertil Suppl. 1999;54:489-97.
Eyal-Giladi H. From Cleavage to Primitive Streak Formation: A Complementary Normal Table and a New Look at the First Stages of the Development of the Chick. 1976;Dev. Biol. 49:321-337.
Furuta et al. Proliferation of exogenously injected primordial germ cells (PGCs) into bulsulfan-treated chicken embryos.Asian J Androl. Dec. 1999;1(4):187-90.
Gagne et al. Electroporation of Bovine Spermatozoa to carry foreign DNA in oocytes. 1991Mol. Reprod. Dev. 29: 6-15.
Gilbert and Wood-Fush, A technique for the fistulation of the hen's oviduct through the abdominal wall, with recovery of the ovum.J. Reprod. Fertil. 1963, 5:451-453.
Godbey, W. et al. Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Apr. 1999,Proc Natl Acad Sci USA96:5177-5181.
Jaenisch R. Retroviruses and Embryogenesis: Microinjection of Moloney Leukemia Virus into Midgestation Mouse Embryos.Cell. Jan. 1980;19(1):181-8.
Lechardeur, D. et al. (1999) Metabolic instability of plasmid DNA in the cytosol: a potential barrier to gene transferGene Ther. 6:482-97.
Li et al. Ballistic transfection of avian primordial germ cell in ovo.Transgenic Research1995, 4:26-9.
Love, et al. Transgenic Birds by DNA Microinjection.Bio/Technology. Jan. 1994; 12:60-63.
Mueller et al. Chimeric pigs following blastocyst injection of transgenic porcine primordial germ cells.Molecular Reproduction and Development. 1999, 54:244-254.
Muramatsu et al. Gene gun-mediated in vivo analysis of tissue-specific repression of gene transcription driven by the chicken ovalbumin promoter in the liver and oviduct of laying hens.Mol Cell Biochem. Aug. 1998; 185(1-2):27-32.
Naito et al. Introduction of exogenous DNA into somatic and germ cells of chickens by microinjection into the germinal disc of fertilized ova.Molecular Reproduction and Development. 1994, 37:167-171.
Naito et al. Production of germline chimeric chickens, with high transmission rate of donor-derived gametes, produced by transfer of primordial germ cells.Mol Reprod Dev. Oct. 1994;39 (2):153-61.
Nakanishi and Iritani, Gene transfer in the chicken by sperm-mediated methods.Molecular Reproduction and Development. 1993, 36:258-261.
Olsen &Neher, The site of fertilization in the domestic fowl.J. Exp. Zoo1948, 109:355-366.
Page et al. “Transgenesis in mice by cytoplasmic injection of polylysine/DNA mixtures”;Transgenic Research, 1995;vol. 4, pp. 353-360.
Pancer et al. Recovery of ova and their re-insertion into the hen's oviduct through a fistula.Br Poult Sci. Dec. 1989; 30(4):953-7.
Phi-Van and Stratling, The matrix attachment regions of the chicken lysozyme gene co-map with the boundaries of the chromatin domain. 1988;E.M.B.O.J.7(3): 655-664.
Pollard H., et al., Polyethylenimine but Not Cationic Lipids Promotes Transgene Delivery to the Nucleus in Mammalian Cells*.J. Biol chem., 1998, 273: 7507-11.
Sang and Perry. Episomal Replication of Cloned DNA Injected into the Fertilised Ovum of the Hen,Gallus domesticus. Mol. Reprod and Devlp.1989, 1:98-106.
Schiest RH, Petes TD. Integration of DNA fragments by illegitimate recombination inSaccharomyces cerevisiae. Proc Natl Acad Sci U S A. Sep. 1, 1991;88(17):7585-9.
Tanaka et al. Chick production by in vitro fertilization of the fowl ovum.J Reprod Fertil. 1994, 100:447-449.
Molecular Structure and Flanking Nucleotide Sequences of the Natural Chick Ovomucoid Gene,Lai et al; Cell 18:829-842 (Nov. 1979).
DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genes, Mandel et al; Nucleic Acids Research 7:2081-2103(1979).
Ovoinhibitor Introns Specify Functional Domains as in the Related and Linked Ovomucoid Gene*, Scott et al; Journal of Biol. Chemistry, 262:5899-5907(1987).
Deoxyribonuclease 1 Sensitivity of the Ovomucoid-Ovoinhibitor Gene Complex in Oviduct Nuclei and Relative Location of CR1 Repetitive Sequences, Scott et al; Biochemistry 26:6831-6840 (1987).
Isolation and characterization of the chicken ovomucoid gene, Lidenmaier et al; Nucleic Acids Research, 7:1

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