Production of a cephalosporin by fermentation

Details Production of a cephalosporin by fermentation Production of a cephalosporin by fermentation

1982-08-06

1985-08-06

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound having a 1-thia-5-aza-bicyclo

435 49, C12P 3500, C12P 3506

Patent

active

045336326

ABSTRACT:
A process for greatly improving the yields of cephalosporin nucleus produced by fermentation is described. Cephalosporin C-producing microorganisms are fermented in the presence of an acetylesterase enzyme so that cephalosporin C formed is immediately converted into desacetyl cephalosporin C before any non-enzymic degradation occurs. Fermentation to produce desacetyl cephalosporin C enables yield increases of cephalosporin nucleus of about 40% to be realized, and greater increases are possible if the fermentation is extended. The preferred cephalosporin C-producing organism is Acremonium chrysogenum, and mutants thereof that are capable of producing esterases in situ. Alternatively, esterases obtained from a variety of other sources may be added or formed in situ, for example from a strain of Rhodosporidium.

REFERENCES:
patent: 3912589 (1975-10-01), Smith
patent: 3926728 (1975-12-01), Kanzaki et al.
Konecny et al., J. of Antibiotics XXVI, No. 3, pp. 135-141 (1973).
Movita et al., Phil. Trans. R. Soc. Lond. B289, pp. 181-190 (1980).
Fujisawa et al., Ag. Biol. Chem. 39(6), pp. 1295-1301 (1975).
Fujisawa et al., Agr. Biol. Chem. 39(6), pp. 1303-1309 (1975).

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