Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2005-03-15
2005-03-15
Bui, Phuong T. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C435S069100, C435S069300, C435S468000, C435S411000, C435S419000, C435S320100, C530S350000, C536S023720, C800S278000, C800S288000, C800S295000, C424S186100, C424S215100
Reexamination Certificate
active
06867353
ABSTRACT:
The present invention relates to a method for producing recombinant rotavirus structural proteins by culturing a transformed plant cell, and to a vaccine composition comprising the same as an effective component. More specifically, the method comprises the steps of preparing the expression vector comprising cDNA fragments encoding rotavirus structural proteins; transforming a plant cell with the expression vector; and recovering the vaccine composition, including rotavirus antigens, from the cell culture. The method for producing the recombinant rotavirus structural proteins is advantageous in that higher yields of more than 0.3 mg/L are obtained, as well as a lower cost and a lower contamination hazard than those of eukaryotic expression systems such as baculovirus and animal cell. Furthermore, the method can be used to produce a edible vaccine. The invention also proves that the rotavirus structural protein can be produced with a high aspect rotary vessel reactor.
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Chung In-Sik
Kim Hong-Joong
Kim Won-Yong
Lee Youn-Hyung
Bui Phuong T.
Burns Doane Swecker & Mathis L.L.P.
Exploregen Inc.
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