Production for recombinant lactoferrin and lactoferrin polypepti

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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43525411, 43525421, 43525423, 4352543, 4353201, 530324, 530350, 530400, 530412, 536 235, 935 10, 935 11, 935 22, 935 28, 935 68, 935 69, C07K 1479, C07K 14435, C12N 1580, C12N 1581

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06100054&

ABSTRACT:
The verified cDNA sequences for human, bovine and porcine lactoferrin protein have been used to prepare recombinant lactoferrin for therapeutic and nutritional applications. Regions of the cDNA such as the Fe binding sites can be used to make an hLF polypeptide product.
The present invention provides novel plasmids, transfected eucaryotic cells and methods of producing these plasmids and transfected eucaryotic cells. The novel plasmid contains the cDNA for lactoferrin protein. Methods for the production of lactoferrin protein in fungi and bacteria are also provided. Thus, the present invention provides an efficient and economical means for the production of recombinant lactoferrin protein and lactoferrin related polypeptides.

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