Chemistry: molecular biology and microbiology – Vector – per se
Patent
1985-11-04
1990-09-25
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Vector, per se
4352523, 43525233, 435 691, 435826, 935 60, 935 65, 935 72, C12N 1563, C12N 1570
Patent
active
049593234
ABSTRACT:
In accordance with the present invention, recombinant plasmids are described which, when inserted into microbial hosts, direct the synthesis of proteins in which preS polypeptides found on the surface of human hepatitis B virus are fused to the enzyme .beta.-galactosidase. The recombinant plasmids are produced by inserting DNA sequences encoding the preS polypeptides into the lacZ gene, which codes for E. coli .beta.-galactosidase, carried by the plasmids. Large amounts of the preS-.beta.-galactosidase fusion proteins can be isolated from microbial cultures carrying the recombinant plasmids. Antigenic determinants of fusion protein so produced are recognized by antibodies to the preS determinants of native hepatitis B virus. The .beta.-galactosidase activity of such fusion protein is detected by a suitable chromogenic or fluorogenic substrate. PreS-.beta.-galactosidase fusion proteins so produced can be used in an enzyme-linked immunosorbent assay (ELISA) to diagnose the presence of hepatitis B virus infection. Additionally, the fusion proteins can be cleaved to release preS polypeptides which can be further purified. PreS polypeptides obtained from these fusion proteins can be used to immunize patients against hepatitis B virus.
REFERENCES:
patent: 4431739 (1984-02-01), Riggs
Wong et al. (1985) CSH Symp. Abstract (May 2-5 1985 Mtg.).
Offensperger et al. (1985) Proc. Nat'l. Acad. Sci. 82: 7540-7544.
Ono et al. (1983) Nucleic Acids REs. 11: 1747-1757.
Heerman et al. (1984) J. Virology 52: 396-402.
Pardee et al., "The Genetic Control and Cytoplasmic Expression of Inducibty" in the Synthesis of .beta.-Galactosidase by E. Coli, J. Mol. Biol (1959), vol. I., pp. 165-168.
Messing, "A Multipurpose Cloning System Based on the Single-Stranded DNA Bacteriophage M13", Recombinant DNA Technical Bulletin, vol. 2, pp. 43-48, (1979).
Casadaban et al., "In Vitro Gene Fusions that Join an Enzymatically Active .beta.-Galactosidase". . . Translational Initiation Signals, Journal of Bacteriology, Aug. 1980, vol. 143, No. 2, pp. 971-980.
Tiollais et al., "Biology of Hepatitis B Virus", Science, vol. 213, Jul. 24, 1981, pp. 406-411.
Neurath et al., "Enzyme-Linked Fluorescence Immunoassays Using .beta.-Galactosidase. . . Polystyrene Plates", Journal of Virological Methods, vol. 3, (1981), pp. 155-165.
Vieira et al., "The pUC Plasmids, an M13mp7-Derived. . . Synthetic Universal Primers", Elsevier Biomedical Press, Gene. vol. 19 (1982), pp. 259-268.
Christman et al., "Amplification of Expression of Hepatitis B. . . Gene and Cloned Viral DNA", Proc. Natl. Acad. Sci. USA, vol. 79, pp. 1815-1819, Mar. 1982.
Casadaban et al., ".beta.-Galactosidase Gene Fusions for Analyzing Gene Expression in Escherichia coil and Yeast", Methods in Enzymology, vol. 100, 1983, pp. 293-309.
Ullman, "One-Step Purification of Hybrid Proteins which have .beta.-Galactosidase Activity", Elsevier, Gene., vol. 29 (1984), pp. 27-31.
Wain-Hobson et al., "Hepatits B Virus", Genetic Maps 1984, vol. 3, Feb. 1984, pp. 92-98.
Neurath et al., "Location and Chemical Synthesis of a Pre-S Gene Coded Immunodominant Epitope of Hepatitis B Virus", Science, vol. 224, Apr. 27, 1984, pp. 392-395.
Newmark, "In Search of Novel Immunogens", Nature, (News and Views) Oct. 8, 1984, pp. 510-511.
Hermann et al., "Large Surface Proteins of Hepatitis B Virus Containing the Pre-S Sequence", Journal of Virology, vol. 52, No. 2, Nov., 1984, pp. 396-402.
Michel et al., "Synthesis in Animal Cells of Hepatitis B . . . Human Serum Albumin", Proc. Natl. Acad. Sci. USA, vol. 81, Dec. 1984, pp. 7708-7712.
Offensberger et al., "Cloning and Expression of the Pre--S1 Region of the Hapatitis B Virus in E. coli", CSH Molecular Biology of HBV, May 2-5, 1985.
Neurath et al., "Hepatis B Virus Contains Pre-S Gene-Encoded Domains", Nature, vol. 315, May 9, 1985, pp. 154-156.
Acs George
Christman Judith K.
Offensperger Wolf
Price Peter
Wahl Silke
Mt. Sinai School of Medicine of the City University of New York
Nolan S.
Schwartz Richard A.
LandOfFree
Production and use of pre S polypeptides of hepatitis B virus does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Production and use of pre S polypeptides of hepatitis B virus, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production and use of pre S polypeptides of hepatitis B virus will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-328867