Production and use of normalized DNA libraries

Details Production and use of normalized DNA libraries Production and use of normalized DNA libraries

1996-06-18

1998-06-09

Elliott, George C.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 6, 435 912, 536 254, C12Q 168, C12N 1500, C07H 2100

Patent

active

057632391

ABSTRACT:
Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample. Also disclosed is a normalized genomic DNA library formed from an environmental sample by the process.

REFERENCES:
Simoens, C. R., et al. 1988. Gene vol. 6 pp. 1-11.

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