Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2002-08-13
2010-10-19
Fronda, Christian L (Department: 1652)
Chemistry: molecular biology and microbiology
Vector, per se
C435S183000, C435S200000, C435S203000, C536S023200
Reexamination Certificate
active
07816129
ABSTRACT:
The present invention is related to an improved production of bacterial proteins in filamentous fungus, e.g. inTrichodermaandAspergillus. The improvement is achieved by constructing expression vectors, which comprise the bacterial protein encoding DNA sequences fused in frame with a DNA sequence encoding a filamentous fungus secretable protein or one or more functional domains of said protein. Filamentous fungus hosts transformed with such expression vectors secrete the desired proteins or enzymes, especially xylanases or cellulases originating from bacteria or more preferably from actinornycetes into the culture medium of the host. The desired proteins or enzymes can be used directly from the culture medium after separation of host cells or recovered and treated using down-stream processes, which are appropriate for the respective application. Xylanases or cellulases from actinomycetes produced using the above expression vectors are most suitable for treating plant derived materials e.g. in pulp and paper industries.
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Fagerström Richard
Lahtinen Tarja
Lantto Raija
Mäntylä Arja
Paloheimo Marja
AB Enzymes GmbH
Fronda Christian L
Sterne Kessler Goldstein & Fox P.L.L.C.
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