Production and recovery of tyrosinase from melanin-synthesizing

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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424115, 435 712, 435101, 435104, 4352521, 5361231, 536126, C12N 902

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054515157

ABSTRACT:
The present invention relates to the discovery of a new bacteria of the genus Alteromonas which has been found to attract oyster larvae by the production of compounds involved in melanin synthesis. More specifically, the present invention contemplates a method for inducing the settlement and metamorphosis of Crassostrea virginica larvae by induction with certain metabolic substances produced by the present bacteria and its altered variants. Furthermore, the present invention is directed to other and derivative metabolic products which can be employed for their desired utility and application. In accordance with the present invention, it has been discovered that ammonia (NH.sub.3) initiates settlement and metamorphosis of the oyster larvae in the same manner as the natural phenomena.

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Yoshida et al. (1973) "Purification of 3,4-Dihydroxylphenyl-L-alanine (L-DOPA) and its Derivatives by Vibrio tyrosinaticus", Arg. Biol. Chem., 37:2121-2126.
Coyne et al. (1989) "Reclassification of Alteromonas colwelliana to the Genus Shewanella by DNA-DNA Hybridization, Serology and 5S Ribosomal RNA Sequence Data," System. Appln. Microbiol., 12:275-279.
Ivins et al. (1980) "Isolation and Characterization of Melanin-Production (mel) Mutants of Vibrio Cholerae" Infec. and Immun., 27:721-729.
Ivins et al. (1981) "Factors Affecting Phaeomelanin Production by a Melanin-Producing (mel) Mutant of Vibrio Cholerae," Infec. and Immun., 34:895-899.
Promerantz et al. (1974) "Purification and Properties of Tyrosinases from Vibrio tyrosinaticus", Arch. Biochem. Biophys., 160:73-82.

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