Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Recovery or purification
Patent
1996-03-11
1997-08-26
Lankford, Blaine
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
Recovery or purification
4352351, 210660, C12N 702
Patent
active
056610238
ABSTRACT:
This invention is directed to a continuous process for producing viral particles by providing in a perfused growth medium a population of viable virally infected non-lytic cells, and removing medium containing said cells at a rate to maintain the steady-state log-phase growth of cells remaining in the perfused growth medium. The invention is also directed to a process for purifying retroviral particles by passing a solution comprising the retroviral particles and contaminants through an anion exchange resin, and eluting the retroviral particles from the resin. The preferred exchange resin is a tentacle anion exchange resin.
REFERENCES:
patent: 3509070 (1970-04-01), Lapidus et al.
patent: 3655509 (1972-04-01), Fields et al.
patent: 3925152 (1975-12-01), Porath et al.
patent: 4647773 (1987-03-01), Gallo et al.
patent: 4725546 (1988-02-01), Sakamoto et la.
Schmid, G. et al., "Continuous Hybridoma Suspension Cultures With and Without Cell Retention: Kinetics of Growth, Metabolism and Product Formation". J. Biotech. 22:31-40 (1992).
Tokashiki, Michiyuki et al., "High Density Culture of Hybridomas Recycling High Molecular Weight Components." Advances in Animal Cell Biology and Technology for Bioprocesses. 355-360 (1989).
Neuman, J.E. et al., "Human T cell Lymphotropic Virus Type III: Affinity Purification of Envelope and Gag Proteins; HTLV-III Protein Isolation and Characterization." Abstr. Ann. Meet. Am. Soc. Microbiol.: (1986) 86meet.324 T-43.
Li, J.K. et al., "Preparative Purification of Viral Polypeptides From Mouse Mammary Tumor Virus." J. Cell Bioche. (1987) Suppl. 11c, 181.
Billich, A. et al., "Purification, Assay and Kinetic Features of HIV-1 Proteinase; HIV virus-1 Recombinant Protease Isolation and Characterization; Gene Cloning and Expression in Escherichia coli." Biol. Chem. Hoppe Seyler; (1990) 371, 3, 265-72.
Rittenhouse, J. et al., "Affinity Purification of HIV-1 and HIV-2 Proteases From Recombinant E. coli strains Using Pepstatin-Agarose; . . . " Biochem. Biophys. Res. Commun. (1990) 171, 1, 60-66.
Nalin, et al., "Purification and Characterization of Recombinant Rev Protein of Human Immunodeficiency Virus Type 1; . . . " Proc. Natl. Acad. Scvi. U.S.A. (1990) 87, 19, 7593-97.
Nalin, C.M. et al., "Purification and Biochemical Characterization of Recombinant HIV-1 Rev Protein; HIV-1 Virus-1 Protein Expression in Escherichia coli." J. Cell. Biochem. (1990) Suppl.14D, 108.
Clarke, J.B. et al., "The Effect of Bioreactor Configuatin on Productin of HIV and Cell-Virus Interaction." Cytotech. 4:145-153 (1990).
Tolbert, William R. et al., "Perfusion Culture Systems for Production of Mammalian Cell Biomolecules." Large-Scale Mammalian Cell Culture. Academic Press, Orlando, Florida 97-119 (1985).
Fabry, L. et al., "High Density Microcarrier Cell Culture for Viral Vaccine Production." Advances in Animal Cell Biology and Technology for Bioprocesses. Butterworth & Co. Ltd. Great Britain 361-365 (1989).
Kloppinger, M. et al., "Flow Cytometric Process Monitoring of Hybridoma Cells in Batch and Perfusion Culture." Advances in Animal Cell Biology and Technology for Bioprocesses. Butterworth & Co. Ltd. Great Britain 125-127 (1989).
Bay Pierre M.
Gore Richard S.
Harter James J.
Hrinda Michael E.
Irish Thomas W.
Lankford Blaine
The Immune Response Corporation
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