Production and purification of biologically active recombinant n

Details Production and purification of biologically active recombinant n Production and purification of biologically active recombinant n

1994-06-27

1997-02-25

Allen, Marianne P.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530402, 530408, 530417, C07K 118, C07K 136, C12N 1512

Patent

active

056060319

ABSTRACT:
A process for the production of biologically active recombinant neurotrophic factor from the NGF/BDNF family is described. The process is comprised of a) constructing a synthetic neurotrophic factor gene suitable for expression in a bacterial expression system; b) the synthetic neurotrophic factor gene is expressed in a bacterial expression system; c) the neurotrophic factor is solubilized and sulfonylated; d) sulfonylated neurotrophic factor is allowed to refold in the presence of polyethylene glycol and urea; and e) biologically active neurotrophic factor is isolated and purified.

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