Product and process for isolating RNA

Details Product and process for isolating RNA Product and process for isolating RNA

1987-11-19

1989-06-27

Griffin, Ronald W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 28, 536 29, C02H 2102

Patent

active

048431559

ABSTRACT:
The present invention discloses a novel method for isolating RNA from biological tissue samples and a novel solvent adapted for use in the disclosed method. The method employs a single extraction using the solvent containing guanidinium and phenol. The solvent is stable for about one month at room temperature without any appreciable phenol oxidation or decomposition. Application of the disclosed method and solvent to a biological tissue sample results in the isolation of a high yield of RNA in a substantially pure and undegraded form. The whole procedure can be completed in three hours, much more quickly than other procedures.

REFERENCES:
patent: 3147185 (1964-09-01), Charney
patent: 3389133 (1968-06-01), Gutcho
R. A. Cox, Methods in Enzynology, 12(B): 120-129, (1968).
J. M. Chirgwin, et al., Biochem 18: 5294-5299 (1979).
J. R. Feramisco, et al., Molecular Cloning, 194-195, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
Kochetkov et al., Organic Chem. of Nucleic Acids, Part A 1971, Plenium Press, pp. 23-28.

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