Product and composition containing a Concholepas concholepas...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C530S857000, C514S012200

Reexamination Certificate

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06916908

ABSTRACT:
The use of CCH-A subunit isolated from hemocyanin of the marine gastropodConcholepas concholepas(CCH), or a combination thereof, as an immunostimulant agent in the innate or adaptive immune response of vertebrates or as an immunotherapeutic agent in cancer of either humans or animals, where the CCH-A subunit has a molecular weight of about 404 kDa, is stable in the absence of Ca+2and Mg+2and is at least 95% pure.

REFERENCES:
patent: 2181-98 (1999-05-01), None
Becker et al., Physico and Immunochemical Study of a New Immunogenic Protein of the Hemocyanin Family From the MolluskConcholepas concholepas(CCH), Jul. 2001, 11th International Congress of Immunolgy, Stockholm Sweden, Abstract, A5.Mon.5.1/1277.
Linn et al., Keyhole Limpet Heamocyanin in Experimental Bladder Cancer, 2000, European Urology, 37(Suppl. 3), 34-40.
Calbiochem catalog, 2000-2001, p. 730, available for public purchase,Concholepas concholepashemocyanin.
R. D. Swerdlow et al., “Immunotherapy with Keyhole Limpet Hemocyanin: Efficacy and Safety in the MB-49 Intravesical Murine Bladder Tumor Model”, The Journal of Urology, Jun. 1994, 1718-1722, vol. 151, American Urological Associations, Inc.
W. Gebauer et al, “Quaternary structure, subunits and domain patterns of two discrete forms of keyhole limpet hemocyanin: KLH1 and KLH2”, Zoology 96 (1994), pp. 51-68, Institute of Zoology, Univ. of Mainz, Germany.
H. Oliva et al., “Monclonal Antibodies to Mulluskan Hemocyanin fromConcholepas concholepasDemonstrate Common and Specific Epitopes among Subunits,” Hybridoma and Hybridomics, vol. 21, No. 5, 2002, Mary Ann Liebert, Inc.

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