Producing pure chondroitinase ABC from Proteus vulgaris ATCC 689

Details Producing pure chondroitinase ABC from Proteus vulgaris ATCC 689 Producing pure chondroitinase ABC from Proteus vulgaris ATCC 689

1995-05-18

1998-06-09

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

210660, 435183, 435188, 435201, C12N 988, C12N 944

Patent

active

057632057

ABSTRACT:
A crystallizable, purified chondroitinase ABC having a molecular weight of about 100,000 dalton by the measurement of the SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and the measurement by the gel permeation chromatography method, having alanine as the N-terminal amino acid and proline as the C-terminal amino acid is disclosed. A process for the purification of the crystallizable purified chondroitinase ABC comprising removing nucleic acid from an surfactant solution extract obtained from cells of chondroitinase ABC-producing microorganisms and chromatographically treating by concentration gradient elution using a weak cation exchange resin or a strong cation exchange resin is disclosed. A composition comprising a chondroitinase and serum albumin, gelatin, or a nonionic surfactant is disclosed. The enzyme is isolated from Proteus vulgaris ATCC 6896.

REFERENCES:
patent: 3549500 (1970-12-01), Suzuki
patent: 4304864 (1981-12-01), Numa et al.
patent: 4623625 (1986-11-01), Scopes
patent: 4696816 (1987-09-01), Brown
patent: 5198355 (1993-03-01), Kikuchi et al.
Japan Patent Application JP 54107587 (Abstract).
Japan Patent Application JP 54107586 (Abstract).
Japan Patent Application JP 73032351 (Abstract).
Japan Patent Application JP 72013704 (Abstract).
Japan Patent Application JP 4330280 (Abstract).
Japan Patent Application JP 62122588 (Abstract).
M. Breen et al., "Microanalysis of Glycosaminoglycans" Anal. Biochem. 113:416-422 (1981).
N. Sato et al., "Depolymerization of Chondroitin C. Sulfate by Immobilized Proteus Vulgaris Cells" Biotech. and Bioeng. 28:1707-1712 (1986).
N. Sato et al., "Cultural Conditions for the Induction of Chondroitinase ABC in Proteus Vulgaris Cells" J. Ferment. Technol. 64(2):155-159 (1986).
N. Sato et al., "Subunit Structure of Chondroitinase ABC from Proteus Vulgaris" Agric. Biol. Chem. 50(4):1057-1059 (1986).

Affiliated with

Also associated with

Rating

Producing pure chondroitinase ABC from Proteus vulgaris ATCC 689 does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Producing pure chondroitinase ABC from Proteus vulgaris ATCC 689, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Producing pure chondroitinase ABC from Proteus vulgaris ATCC 689 will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2197778