Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...
Patent
1997-08-26
2000-07-25
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
435 2, 435 4, 435 41, 435325, 435243, 4352831, 4352891, 4353071, 436547, 530300, 530350, 536 221, C12N 900, G01N 3353, C07K 1700, C07H 2100
Patent
active
060935596
ABSTRACT:
Hydrophobic polymer surfaces whose level of protein binding is less than about 50-80 ng/cm.sup.2 are achieved by: (1) applying to a hydrophobic polymer surface a coating solution composed of a solvent and a non-ionic surfactant having a HLB number of less than 5 and at least one hydrophilic element which can extend into an aqueous solution; and (2) drying the surface to remove the solvent and thereby bring the surfactant into direct contact with the hydrophobic polymer. The combination of a low HLB number and the drying step have been found to produce low binding surfaces which can withstand multiple washes with water and/or protein-containing solutions. Alternatively, the low binding surfaces can be produced by applying the non-ionic surfactant to mold surfaces which contact molten polymer and form the polymer into a desired shape, e.g., into a multi-well plate, a pipette tip, or the like. Further, the low binding surfaces may be produced by incorporating non-soluble, non-ionic surfactants having an HLB number of less than or equal to 10 into a polymer blend prior to molding the article.
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Bookbinder Dana Craig
Fewkes, Jr. Edward John
Griffin James Arthur
Smith Frances M.
Tennent David L.
Beall Thomas R.
Corning Incorporated
Naff David M.
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