Producing lipid-protein membranes for chemical detection

Details Producing lipid-protein membranes for chemical detection Producing lipid-protein membranes for chemical detection

1984-06-20

1987-04-28

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 7, 435174, 436501, 436528, 436806, 530812, G01N 33531, G01N 33544

Patent

active

046614427

ABSTRACT:
Stable lipid-protein membranes are prepared by providing a medium of dissolved and emulsified lipids and dissolved proteins. The medium is applied to an orifice formed in a sheet of material in sufficient volume to provide sufficient lipid and protein to form a membrane across the orifice, which membrane comprises a bimolecular lipid layer with protein molecules interspersed therein. The medium is allowed to dry in the presence of air, and the lipid and protein molecules arrange themselves to form the membrane. The protein is selected to be specifically reactive with a particular chemical substance or a narrow class of chemical substances. When the membrane is exposed to a substance with which the membrane proteins react, a physical characteristic of the membranes is altered. Detection of a change in such membrane characteristic confirms the presence of the substance.

REFERENCES:
J. Del Castillo et al., Science 153, 185-188, 1966.
M. Mio et al., Adv. Biosci. 33, 7-23, 1982.
Y. Rovin in N. Kovarskii (Ed.) Bisloinye Lipidyne Membr., 1983, pp. 3-17, [cited in Chem. Abs. 101, 35254s, 1984].
Janos H. Fendler C&EN Jan. 2, 1984, pp. 25-38.
Janos H. Fendler Membrane Mimetic Chemistry John Wiley & Sons, New York 1982.

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