Processing plant material with xylanase

Details Processing plant material with xylanase Processing plant material with xylanase

1996-09-23

1999-02-23

Prats, Francisco

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 72, 435 99, 435277, 435274, 435209, C12N 924, D21C 100, C12P 1914

Patent

active

058742743

DESCRIPTION:

BRIEF SUMMARY
CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a 35 U.S.C. 371 national application of PCT/DK95/00082 filed Feb. 24, 1995, which is incorporated herein by reference.


FIELD OF THE INVENTION

The present invention relates to the use of a xylanase preparation for reducing the viscosity of a plant material and for separating a plant material, such as wheat, into separate useful components as well as processes for such viscosity reduction or separation.
BACKGROUND OF THE INVENTION
Wheat contains valuable components of gluten and starch which can be recovered in wet milling processes. Vital gluten is the primary product, whereas high quality A-starch and low quality B-starch are valuable by-products. Residual fibre and soluble solids are further by-products.
The commercially available Trichoderma reesei cellulase enzyme preparation, Spezyme.RTM. CP (Genencor, USA) has been suggested for lowering the viscosity and improving wheat and corn starch processing.
Weegels et al., (1992), describe the use of enzymes (cellulase, hemicellulase, lipase and protease/amylase) in a wheat separation process. It is concluded that cellulases and hemicellulases improve the processing properties of wheat and increase the yield of gluten and starch. The hemicellulase used was a xylanase preparation containing significant side-activities including amylase and protease activity.
It is known that xylanases are capable of degrading wheat flour and other plant derived materials into a number of different degradation products. Xylanases purified from a strain of the fungal species Aspergillus awamori have been described in a number of references, c.f., e.g. Kormelink et al., (1993) disclosing physicochemical and kinetic characteristics of three A. awamori endo-xylanases. The degradation products obtained by use of such A. awamori xylanases in the enzymatic degradation of various plant materials including rice bran, oat spelts, wheat flour, larch wood and birch wood are described by, inter alia, Kormelink et al., (1991), Kormelink et al., (1992), J. M. Kormelink and A. G. J. Voragen (1993), Gruppen et al., (1992), and Gruppen et al., Symposium October 1993.
Voragen et al., (1992), and Gruppen et al., (1993a and 1993b) disclose the degradation of water-extractable and water-unextractable arabinoxylan fractions isolated from wheat flour with A. awamori endo-xylanases. The latter reference describes the use of two of the A. awamori xylanases in the baking of wheat loaves.
Shei et al., (1985), and Fournier et al., (1985), describe purification and characterization of endoxylanases isolated from A. niger.
None of the above references mentions that xylanases may be used in separation of wheat nor how to select xylanases of particular use for said purpose.
Dusterhoft et al., Symposium October 1993, describe the use of endoxylanase in degradation of non-starch polysaccharides in animal feed and Vietor et al., (1993), the use of endoxylanase in reducing the viscosity of wort during the beer brewing process.
It is an object of the present invention to provide improved processes for modifying the viscosity of a plant material and for separation of a plant material into desirable components.


BRIEF DESCRIPTION OF THE DRAWING

FIG. 1 shows the specific viscosity of xylanase II combined with other enzymes.
FIG. 2 shows the principle in the wet milling wheat separation process.
FIG. 3 shows the AMEn of wheat in Broilers. BF+=Bio-Feed.RTM. Plus Xyl II=Xylanase II


BRIEF DISCLOSURE OF THE PRESENT INVENTION

The present inventors have now surprisingly found that the activity of xylanase on water soluble and water insoluble pentosans, respectively, seems to be important for the efficiency of the xylanase in reducing the viscosity and/or separation of components of a plant material. The present invention is based on this finding.
Accordingly, in a first aspect the invention relates to the use of a xylanase preparation for modifying the viscosity of a plant material, in which the xylanase preparation exhibits
In a second important aspect the

REFERENCES:
patent: 4144354 (1979-03-01), Unno et al.

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