Processes of producing amylase inhibitors

Details Processes of producing amylase inhibitors Processes of producing amylase inhibitors

1994-08-05

1995-08-08

Russel, Jeffrey E.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Plant proteins, e.g., derived from legumes, algae or...

530375, 530415, C07K 132, C07K 14415

Patent

active

054400198

ABSTRACT:
A process of producing an amylase inhibitor from an amylase inhibitor-containing solution extracted from wheat flour, etc with water, a dilute acid, a dilute alkali or from an amylase inhibitor-containing starch waste solution, by utilization of an adsorption of the amylase inhibitor on a calcium phosphate gel, while removing impure proteins contained in the solution. The process can produce in economy and high yields the amylase inhibitor having a very high amylase inhibitory activity but no or very little trypsin inhibitory activity.

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Journal of Food Biochemistry, vol. 1, 1977, pp. 385-401, Gramm, et al., "Purification and Characterization of Alpha-Amylase Inhibitors in Wheat".
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Eur. J. Biochem, 183, Rosa Sanchez-Monge, et al., "New Dimeric Inhibitor of Heterologous .alpha.-Amylases Encoded by a Duplicated Gene in the Short Arm of Chromosome 3B of Wheat (Triticum Aestivum L)", 1989, pp. 37-40.
Phytochemistry, vol. 20, No. 8, Nizar Kashlan, et al., "The Complete Amino Acid Sequence of a Major Wheat Protein Inhibitor of .alpha.-Amylase", 1981, pp. 1781-1784.

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